A method for producing monoclonal antibodies to human oral epithelial cells by the hybridoma technique.
Female BALB/c mice 6-8 weeks old were immunized intravenously (IV) with two injections of 30,000 human oral epithelial cells two weeks apart. The sera of these mice, and none of the controls, were positive by indirect immunofluorescence to cell suspensions of human epithelial cells of gingivae, tonsils and skin, but were negative to sections of human spleen, kidney and liver. The fluorescence was apparent only in the prickle cell layer, not at the basement membrane region nor in the connective tissue. The spleen cells from one of the selected immunized mice were fused to mouse myeloma cells, using polyethylene glycol (1500 MW), three days after a third IV booster injection of 30,000 epithelial cells. Three of the monoclonal cell lines produced have shown different staining patterns with various sizes of epithelial cells in suspension, results that differ from the staining pattern obtained with epithelial cells using polyclonal sera. These monoclonal antibodies were directed against antigens that differ from blood group A, B and HLA antigens on the surface of epithelial cells.[1]References
- A method for producing monoclonal antibodies to human oral epithelial cells by the hybridoma technique. Gazi, M.I. Journal de biologie buccale. (1986) [Pubmed]
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