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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Net nutrient absorption in beef steers fed silage or high concentrate diets containing four levels of limestone.

Net absorption of ammonia N, urea N, glucose, L-lactate, and volatile fatty acids was measured in four Hereford x Angus steers fed ad libitum orchard grass-clover silage. Net absorption was calculated as the product of blood plasma flow from portal-drained viscera and the difference in concentration between plasma from the portal vein and femoral artery. Measurements were made at 15-minute intervals for 4 hours. The steers were then adapted to four 92% concentrate diets containing 0.64, 1.42, 2.21, or 3.00% limestone. Net absorption measurements were repeated in a 4 x 4 Latin square design. Portal blood flow was slower (P less than 0.05), net ammonia N absorption was greater (P less than 0.05), and net absorption of L-lactate and propionate was lesser (P less than 0.10) when steers were fed silage than when they were fed 92% concentrate. There was net loss of urea N and glucose from plasma to the gut regardless of diet. Dietary limestone level did not affect net absorption rates of any nutrient measured except L-lactate, which decreased (P less than 0.10) as limestone level in the diet increased.[1]


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