Improved method of enrichment for immature myeloid cells from normal human bone marrow.
A method of enrichment for immature myeloid cells from normal human bone marrow has been described. The method is based on 4 consecutive steps: 1. Density cut centrifugation. After centrifugation all cells above the pellet were collected. This suspension contained 93% of the originally present myeloblasts and promyelocytes. The majority of normoblasts and granulocytes was found in the pellet. 2. Nylon wool filtration. This procedure was performed to remove the majority (87%) of the monocytes. Recovery of myeloblasts and promyelocytes after filtration was 74%. 3. Centrifugation on a continuous density gradient. This procedure resulted in an additional purification of the myeloblasts and promyelocytes within a specific fraction. By centrifugation the concentration of myeloblasts and promyelocytes was increased from 19% to 38%. 4. Velocity sedimentation at 1 g. This technique produced a 82% pure immature myeloid cell suspension, comprising 57% myeloblasts and promyelocytes. All figures are the mean of 10 experiments.[1]References
- Improved method of enrichment for immature myeloid cells from normal human bone marrow. Burghouts, J., Plas, A.M., Salden, M., Wessels, J. Exp. Hematol. (1980) [Pubmed]
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