The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Asymmetrical distribution and artifactual reorientation of lipopolysaccharide in the outer membrane bilayer of Salmonella typhimurium.

Labelling of cell walls or outer membranes from Salmonella typhimurium with ferritin-conjugated antibodies directed against the polysaccharide moiety of the lipopolysaccharide gave the following results: 1. Cell walls or outer membranes from which the mucopeptide had been removed by lysozyme digestion at 0 degrees C carried the label on the outer face of the membrane. 2. When the murein layer was removed by either lysozyme or trypsin at physiological temperature (25-37 degrees C) subsequent labelling showed the lipopolysaccharide to be present on both membrane faces. 3. This reorientation could be achieved by a 1-min treatment of the membranes at 37 degrees C. 4. Glutaraldehyde fixation of the outer membranes did not entirely prevent but somewhat inhibited the temperature-induced reorientation process. 5. The same reorientation phenomenon was observed in lysozyme spheroplasts, which were prepared at 37 degrees C and were subsequently lysed in hypotonic medium at 0 degrees C. These observations are discussed as evidence for a transmembrane movement of lipopolysaccharide, which only takes place in areas where the mucopeptide layer is defective, and only when the temperature is sufficiently high to allow such movement.[1]


WikiGenes - Universities