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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Molecular cloning and expression of the gene encoding human angiotensin II type 2 receptor.

The gene of human angiotensin II type 2 (AT2) receptor was isolated from a genomic DNA library prepared from human placenta. The coding region of the human AT2 receptor gene was contained in a single exon coding segment of the gene indicating an intronless structure of the coding region. The amino acid sequence of human AT2 receptor deduced from its nucleotide sequence has 363 amino acids and shows a high degree of sequence identity to rat and mouse receptor sequences. Specific binding of [125I]Sar1Ile8-angiotensin II was demonstrated in COS-7 cells transfected with a plasmid containing the human AT2 sequence. Scatchard analysis and ligand displacement profile were typical of the AT2 receptor. Reverse transcription-polymerase chain reaction analysis showed that AT2 receptor mRNA was expressed in adult uterus and pheochromocytoma.[1]

References

  1. Molecular cloning and expression of the gene encoding human angiotensin II type 2 receptor. Tsuzuki, S., Ichiki, T., Nakakubo, H., Kitami, Y., Guo, D.F., Shirai, H., Inagami, T. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
 
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