Androgen binding proteins in Cochliobolus lunatus.
Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min at 0 degrees C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean Kd determined for testosterone binding protein ( TBP) by Scatchard analysis was 4.5 nM and the maximum capacity Bmax was 120 fmol/mg protein; for androstenedione binding protein ( ABP) the Kd was 8 nM and Bmax was 390 fmol/mg protein. Steroid binding specificity for TBP, in order of decreasing affinity, was the following: androstenedione, progesterone, dihydrotestosterone, 17 beta-estradiol and estrone. The same order was found for ABP.[1]References
- Androgen binding proteins in Cochliobolus lunatus. Kastelic-Suhadolc, T., Lenasi, H. FEMS Microbiol. Lett. (1993) [Pubmed]
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