Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Robinson, M.L. et al.

Differential expression of alpha A- and alpha B-crystallin during murine ocular development.

PURPOSE: To compare the temporal and spatial expression patterns of alpha A- and alpha B-crystallin mRNA during ocular development. METHODS: Tissue samples from embryonic day 9.5 (E9.5) through postnatal day 14 were collected from FVB/N strain mice. The specimens were fixed in paraformaldehyde, histologically processed, and assayed for alpha A- and alpha B-crystallin mRNA expression by in situ hybridization. RESULTS: During ocular development, alpha B-crystallin transcripts are present in the lens placode at E9. 5. Transcripts of alpha A-crystallin are first observed in the lens cup at E10 to 10. 5. During subsequent development of the lens, alpha A crystallin transcripts are most abundant in the fiber cells, and alpha B crystallin mRNA is preferentially expressed in epithelial cells. Transcripts of alpha A-crystallin were detected only in the lens. In contrast, alpha B-crystallin transcripts are present in retinal pigment epithelium, optic nerve, extraocular muscle, iris, ciliary body, cornea, and several nonocular sites, such as heart and nasal epithelium. CONCLUSIONS: Transcription of alpha B-crystallin precedes the expression of alpha A-crystallin during murine ocular development. Furthermore, the patterns of alpha A- and alpha B-crystallin expression in the lens are distinctive: alpha A is upregulated and alpha B is downregulated during prenatal fiber cell differentiation. These results indicate that the alpha-crystallin genes are not identically regulated either within or outside the lens.[1]

References

Differential expression of alpha A- and alpha B-crystallin during murine ocular development. Robinson, M.L., Overbeek, P.A. Invest. Ophthalmol. Vis. Sci. (1996) [Pubmed]

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