Disease relevance of Cobalt muriate

• To further understand the regulation of Epo gene expression, we studied the effects of hypoxia and CoCl2 on the rate of Epo gene transcription [1].
• The human hepatoma cell line, Hep3B, synthesizes large quantities of erythropoietin (Epo) mRNA and protein in a regulated manner in response to hypoxia and cobaltous chloride (CoCl2) [1].

High impact information on Cobalt muriate

• The extent of heme oxygenase induction by CoCl2 was also increased about 2-fold above normal in adrenalectomized animals and was accompanied by an enhanced breakdown of cytochrome P-450 [2].
• Cobalt dichloride was used to induce erythropoietin, a growth factor for erythropoiesis [3].
• An increased induction of MPCE in the bone marrow by two chemicals--benzo(a)pyrene, 2-naphthylamine--was also observed following pretreatment with cobalt dichloride [3].
• Increased sensitivity of the micronucleus test was attributable to the administration of mutagen during the differentiation and multiplication of erythroblast, which is presumed to have been accelerated by pretreatment with cobalt dichloride [3].
• The greatest interaction between cobalt pretreatment and mutagen treatment for the induction of micronucleated polychromatic erythrocytes (MPCE) occurred when mice were injected with 1,1-dimethylhydrazine (DMH) 12-24 hr after pretreatment with cobalt dichloride and killed 30 hr later [3].

Anatomical context of Cobalt muriate

• Acute lesion of rat myocardium by a single injection of cobalt dichloride [4].

Analytical, diagnostic and therapeutic context of Cobalt muriate

• While Northern blot analyses showed that steady-state Epo mRNA levels increase more than 50-fold in response to hypoxia or CoCl2, nuclear run-off experiments demonstrated only a 10-fold increase in Epo gene transcription in response to these stimuli [1].
• The formation of infarctoid areas in rat myocardium following intraperitoneal injection of cobalt dichloride (30 mg/kg) is connected with metabolic (toxic) and ischaemic lesions of myocardial cells [4].

References