High impact information on Dibromo-bapta

• We have investigated the role of Ca2+ in the excitation of Limulus photoreceptors by intracellular injection of the Ca2+ buffer, 5,5'-dibromo-BAPTA [1].
• We further investigated the effect of reducing any [Ca2+]i gradients by microinjecting dibromo-BAPTA into the blastomere [2].
• Dibromo-BAPTA injection completely blocked mitotic cell division when a final concentration of 1.5 mM was used [2].
• When Calcium was introduced into the cell with pipette-loaded dibromo-BAPTA, elevation of [Ca2+]i into the range 1-10 microM induced membrane capacitance increases indicative of exocytosis in a concentration-dependent manner [3].
• Microinjection of the Ca2+ buffer 5,5'-dibromo-BAPTA before cold shock, blocked cytokinesis suggesting that the transient rise in [Ca2+]i may be necessary for induction [4].

Biological context of Dibromo-bapta

• The introduction of an appropriate concentration of the weak (KD = 1.5 micromol/L) calcium buffer 5,5'-dibromo-BAPTA results in the dissipation of these calcium domains, and inhibits cytoplasmic streaming and the subsequent formation of a BD at the animal pole [5].

Gene context of Dibromo-bapta

• Treatment with dibromo BAPTA/AM, an intracellular calcium buffer, blocked SP-induced IL-1 production [6].

Analytical, diagnostic and therapeutic context of Dibromo-bapta

• The subsequent gradual increase in [Ca2+]i may not be critical because microinjection of 5,5'-dibromo-BAPTA during cleavage plane development did not always perturb division [4].

References