High impact information on Cytoplasmic Streaming

• It is involved in various cellular processes such as motility, cytoplasmic streaming, chromosome segregation and cytokinesis [1].
• Premature microtubule-dependent cytoplasmic streaming in cappuccino and spire mutant oocytes [2].
• By inducing cytoplasmic streaming in wild-type oocytes during mid-oogenesis, we show that premature cytoplasmic streaming can displace staufen protein from the posterior pole, but not gurken mRNA from around the oocyte nucleus [3].
• On the other hand, cytoplasmic streaming in pollen tube was inhibited by either N-ethylmaleimide or heat treatment [4].
• In conclusion, cytoplasmic streaming in pollen tubes is suggested to be regulated by Ca2+ through "myosin" inactivation [4].

Anatomical context of Cytoplasmic Streaming

• Injection of active BAPTA-type buffers or application of elevated levels of sucrose reversibly inhibited growth, destroyed tip zonation of organelles, and modified normal patterns of cytoplasmic streaming [5].
• Microinjection of the antiserum against P-115-ABP into living root hair cells caused the disappearance of transvaculor strands and alteration of the route of cytoplasmic streaming [6].

Associations of Cytoplasmic Streaming with chemical compounds

• When 5% dimethyl sulfoxide (DMSO) was applied to Dictyostelium cells, the cells rounded up in shape and cytoplasmic streaming ceased [7].
• EGTA at appropriate concentration could induce cytoplasmic streaming in these cells [8].
• The vitality of the cells after nanoprocessing has been ascertained by exclusion of propidium iodide from the cells as well as by the presence of cytoplasmic streaming [9].
• It is well known that the cytoplasmic streaming of characean cells is readily inhibited by Ca(2+) [10].
• In mesophyll cells of the aquatic angiosperm Vallisneria gigantea Graebner, a rapid and transient inhibition of cytoplasmic streaming was induced by hypertonic treatment with sorbitol [11].

Gene context of Cytoplasmic Streaming

• We also show that capu mutant oocytes have abnormal microtubule distributions and premature microtubule-based cytoplasmic streaming within the oocyte, but that neither the speed nor the timing of the cytoplasmic streaming correlates with the strength of the mutant allele [3].
• Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte [12].
• We propose that, in mei-1 mutant oocytes, this cytoplasmic streaming is sufficient to drive the spindle into the cortex [13].
• Furthermore, yps mutants partially suppress the premature cytoplasmic streaming of orb mutants [14].
• Surprisingly, cytoplasmic streaming still occurs in kinesin light chain null mutants, and both oskar mRNA and Dynein localise to the posterior pole [12].

References