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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


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Disease relevance of Microinjections


Psychiatry related information on Microinjections


High impact information on Microinjections


Chemical compound and disease context of Microinjections


Biological context of Microinjections


Anatomical context of Microinjections


Associations of Microinjections with chemical compounds

  • Expression of cDNA constructs and microinjection of PLC or antibodies against it clearly establish a role for PtdIns signaling distinct from its role in calcium mobilization and protein kinase C activation [31].
  • Local application of the GABA agonist muscimol prevents the appearance of seizures on subsequent microinjection of all convulsant agents examined, whereas local application of the muscarinic antagonist, atropine, only prevents seizures induced by carbachol [32].
  • As microinjection of antibodies against p21ras can block the biological effects of both normal and oncogenic tyrosine kinases, it is likely that they require functional p21ras to transmit their mitogenic signals [33].
  • Global perturbation of the extracellular matrix by microinjection of Arg-Gly-Asp peptides or heparinase into the blastocoel resulted in global randomization of left-right asymmetries [34].
  • Blockade of gamma-aminobutyric acid receptor function by direct microinjection of bicuculline into the nucleus ambiguous in cats produced a marked increase in gastric motility which was mediated by the vagus nerve [35].

Gene context of Microinjections

  • To examine whether GRB2 is also a component of ras signaling in mammalian cells, microinjection studies were performed [36].
  • Expression of a dominant negative form of Spred and Spred-antibody microinjection revealed that endogenous Spred regulates differentiation in these types of cells [37].
  • Furthermore, microinjection of dominant negative forms of Rac and Cdc42 or of the Rho inhibitor C3 transferase blocked serum-induced DNA synthesis [38].
  • Here we performed microinjection and transfection experiments using rat R12 fibroblasts, their derivatives conditionally overexpressing cyclins D1 or E, and human U-2-OS cells, to explore the action of G1 cyclins and the relationship of E2F and cyclin E in S-phase induction [39].
  • Lastly, a significant decrease in anti-IgM-mediated apoptosis was seen upon downregulation of endogenous p53 activity by expression of a dominant-negative p53 protein or upon microinjection of an antisense p21 expression vector or antibody [40].

Analytical, diagnostic and therapeutic context of Microinjections


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