Disease relevance of Chloromycetin

• Transient transfection of H4IIE hepatoma cells and 3T3-30A5 preadipocytes with plasmids containing the chloroamphenicol acetyltransferase gene driven by FAS promoter sequences of different lengths revealed that two regions between nucleotides -249 and -30 contain elements capable of enhancing transcription [1].
• A single injection of a long-acting oily preparation of chloramphenicol (Tifomycine) was compared with a five-day course of crystalline and procaine penicillin in the treatment of 131 adult patients with meningococcal meningitis [2].
• Protein synthesis by both laboratory-grown bacteria and isolated nodule bacteroids of Rhizobium lupini (strain WU8) is inhibited by D-threo-chloramphenicol, the bacteroid form being the more sensitive to the antibiotic [3].
• Following the construction of a series of pSV2-cat derived plasmids containing the chloramphenicol acetyltransferase (CAT) gene under the control of a eukaryotic trout protamine promoter, it was noted that Escherichia coli, transformed with these plasmids, developed resistance to chloramphenicol (CM) [4].

High impact information on Chloromycetin

• Protein synthesis by isolated mitochondria of resistant cells was found to be less inhibited by concentrations of both Tevenel and D-chloramphenicol up to 200 mug/ml than the protein synthesis by LMTK- mitochondria [5].
• Treatment of FLG 29.1 cells with 10 nM 17 beta E2 increased chloroamphenicol acetyltransferase expression from 5- to 29-fold compared to controls [6].
• We now report that differentiation represses SRE serum inducibility using nontransformed cells transiently transfected with pjunB SRE thymidine kinase/chloroamphenicol acetyltransferase (SREtk/CAT) or pc-fos SREtk/CAT containing an intact SRF-binding domain [7].
• Using deletion mutants of the 5'-flanking region and a linked reporter (the bacterial chloroamphenicol transferase gene), we have investigated the elements of the calcyclin gene's promoter that respond to growth factors [8].
• In contrast, a transiently transfected MMTV chloroamphenicol acetyl transferase reporter, while much more inducible by glucocorticoids, can be induced significantly by progestins [9].

Biological context of Chloromycetin

• In transient transfection assays using several cell lines, little or no stimulation of alpha 1(I)-driven chloroamphenicol acetyltransferase transcription was observed [10].
• Chloroamphenicol acetyltransferase-reporter assays revealed a functional CpG-rich promoter/enhancer region located between nucleotide -621 and the major transcriptional initiation site [11].
• Quinoline-grown cells of strain 86 treated simultaneously with 2-chloroquinoline and D-(-)-threo-chloramphenicol to prevent protein biosynthesis also formed the cis-7,8-dihydrodiol of 2-chloroquinoline [12].
• In about 20% of the cases, when the original sperm motility was low, it was found that 40 micrograms ml-1 D-chloramphenicol has maximum stimulation effect on sperm motility in inverse fashion [13].
• Modification of the trout protamine promoter region by removing the region containing the eukaryotic Goldberg-Hogness box in the plasmid p525-cat increased the expression of the CAT gene almost to the wild-type level and conferred strong CM resistance [4].

Anatomical context of Chloromycetin

• Isolation and detailed characterization of human cell lines resistant to D-threo-chloramphenicol [14].
• At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa [13].
• The activity of monoamine oxidase, a marker enzyme of the outer mitochondrial membrane, is stimulated in BHK cells grown in the presence of 100 microgram ml-1 D-chloramphenicol [15].

Associations of Chloromycetin with other chemical compounds

• Cycloheximide and chloroamphenicol, specific inhibitors of protein translation in the cytoplasmic and cyanellar compartments, respectively, of Cyanophora paradoxa, have been employed in 30 min pulse-labelling experiments by using [NaH-14C]O3 to label total cell proteins in vivo [16].

Gene context of Chloromycetin

• 2,4-, 3,5- and 3,4-DCPSO2Mes increased the activity of UDPGT toward CP but not toward p-NP [17].
• Amphenicol and Macrolide Derived Antibiotics Inhibit Paraoxonase Enzyme Activity in Human Serum and Human Hepatoma Cells (HepG2) in vitro [18].

References