High impact information on Rhodamine 123

• Combination of the two agents, CDDP+caffeine, strongly suppressed not only cell viability but also R123 uptake and cell size, compared with CDDP pretreatment alone [1].
• Cell cycle arrest in G2 + M phase and a concomitant increase in both rhodamine 123 (R123) uptake and cell size (forward scatter) were observed in these cells [1].
• Binding to mitochondria was temperature-dependent and the degree of binding was in the order of TMRE > R123 > TMRM [2].
• A direct concentration-effect relationship between zosuquidar.3HCl concentrations and inhibition of rhodamine 123 (Rh123) efflux in CD56 lymphocytes was defined by a sigmoid E(max) model [3].
• In contrast, the strongest R123 fluorescence signal is detected in the intracellular cytoplasm of the viable epidermal keratinocytes [4].

Biological context of Rhodamine 123

• In the AUC method, two samples with identical R123 brightness or percentage below cutoff after dye efflux can have very different total activities, depending on their basal activity [5].

Anatomical context of Rhodamine 123

• By using rhodamine 123 (Rh123) monitored by flow cytometry in living hepatocytes, we were able to follow in time delta psi in such DNP-uncoupled cells incubated with various substrates [6].
• Intracellular oxidation of dihydrorhodamine 123 (DHR) to the fluorescent compound rhodamine 123 (Rho123) was used to detect the production of oxygen metabolites in activated neutrophils [7].

Gene context of Rhodamine 123

• P-gp activity can be detected by measuring efflux of fluorescent substrates such as rhodamine 123 (R123) [5].

References