Disease relevance of LASS1

• Using a baculovirus vector expression system, we found that a 10 kDa recombinant LAG-1 protein is secreted by AcNPV infected SF9 cells, as determined in Western blot experiments by the reactivity of specific anti-peptidic heteroantibodies [1].

High impact information on LASS1

• Homologues of the Lag 1 protein can be found throughout phylogeny, although sequence similarity is very limited [2].
• The Lag 1 protein is located in the endoplasmic reticulum (ER), where it helps to accelerate the transport of glycosylphosphatidylinositol (GPI)-anchored proteins to the Golgi [2].
• Finally the natural LAG-1 protein was precipitated from the supernatant of internally labeled activated Nk cells and shown to migrate as a single entity of 14 kDa in SDS-PAGE analysis [1].
• Cloning and sequence analysis revealed that this transcript contains two nonoverlapping open reading frames (ORFs), LASS1 and GDF1, which are quite different to the predicted sequences in GenBank (accession number XM_224734 and XM_224733) [3].
• To explore the potential role of LASS1 in rat neuron aging, northern blot analysis for LASS1 mRNA was performed and detected a 2.7 kb transcript in adult rat cerebral cortex [3].

Other interactions of LASS1

• LASS2, containing the conserved TLC domain and the specific HOX domain, is a human homologue of Lag1p [4].
• Molecular cloning, characterisation and tissue-specific expression of human LAG3, a member of the novel Lag1 protein family [5].

References