Disease relevance of EPAG

• We have isolated a novel 667-bp cDNA clone, designated epag, from a Hodgkin's-disease cell line-derived library that is expressed in association with T cell activation and which is not related to any known gene family [1].

High impact information on EPAG

• Epag expression was detected by reverse transcription/PCR in RNA preparations made from various normal nonlymphoid tissues [1].
• We hypothesize that epag functions as an early signal that helps mediate the activation of T cells [1].
• Sequence analysis of multiple independent cDNA clones showed that epag expressed in the Hodgkin's-disease cell line L428 is identical to the gene expressed in normal activated PBMC [1].
• Expression of epag was also detected in PMA- and PHA-stimulated, but not in nonstimulated Jurkat cells, and overall its expression in transformed cell lines of hemopoietic origin is highly restricted [1].
• The lymphoid proliferation-associated gene EPAG maps to human chromosome region Xq21-->q22 [2].

Biological context of EPAG

• Using Southern blot and fluorescence in situ hybridization analyses, we have shown that the novel human gene EPAG, expressed in association with the mitogenic activation of T and B cells in peripheral blood, maps to chromosome region Xq21-->q22 [2].

Analytical, diagnostic and therapeutic context of EPAG

• By using reverse transcription/PCR, we have demonstrated that epag mRNA is expressed as early as 1 h after stimulation of normal PBMCs with anti-CD3 [1].

References