Disease relevance of NUPL2

• An interaction between HIV-1 Vpr and the human nucleoporin CG1 (hCG1) was revealed in the yeast two-hybrid system, and then confirmed both in vitro and in transfected cells [1].
• NLP-1 represents a lead compound for intercalating 2-nitroimidazoles with selective toxicity for hypoxic cells [2].

High impact information on NUPL2

• Using a nuclear import assay based on digitonin-permeabilized cells, we demonstrate that hCG1 participates in the docking of Vpr at the nuclear envelope [1].
• In addition, gene expression analysis and wheat germ agglutinin chromatography experiments suggested that NLP-1 is an ubiquitous O-glycosylated nuclear protein [3].
• This protein has features found in nucleoporins including many phenylalanine-glycine repeats, a very high serine content, a putative zinc finger, and a coiled-coil domain; we thus called it NLP-1 (nucleoporin-like protein 1) [3].
• CG1 is composed of a gadolinium contrast agent core tethered to copper-selective recognition motif [4].
• Mutation analysis of the CG1 gene in DFNA5 patients, however, could not reveal a disease-causing mutation [5].

Chemical compound and disease context of NUPL2

• At a concentration of 0.5 mM, NLP-1 is 8 times more toxic to hypoxic than aerobic cells at 37 degrees C. This concentration is 40 times less than the concentration of misonidazole, a non-intercalating 2-nitroimidazole, required for the same degree of hypoxic cell toxicity [2].

Anatomical context of NUPL2

• We cloned a novel candidate gene (CG1, candidate gene 1), which is expressed in human fetal cochlea, from the DFNA5 candidate region [5].

Associations of NUPL2 with chemical compounds

• The 2-nitroimidazole linked phenanthridine, NLP-1 (5-[3-(2-nitro-1-imidazoyl)-propyl]-phenanthridinium bromide), was synthesized with the rationale of targeting the nitroimidazole to DNA via the phenanthridine ring [2].

References