Disease relevance of nos

• We have characterized a tobacco nuclear factor that binds to the -118 region of the nopaline synthase (nos) promoter from the Ti plasmid of Agrobacterium tumefaciens [1].
• Chimeric genes composed of DNA complementary to alfalfa mosaic virus (AIMV) RNAs 1 or 2, the CaMV 35 S promoter, and the nos polyadenylation signal were transferred to the genome of Nicotiana tabacum cv. Samsun NN by means of the Agrobacterium tumefaciens transformation system [2].

Biological context of nos

• The method employs an inverted repeat of the 3'-untranslated region (3'-UTR) of a heterologous gene, and has been demonstrated using the 3'-UTR region of the nopaline synthase (nos) gene from Agrobacterium tumefaciens, which is often used as the 3'-UTR for transgene constructs [3].

Analytical, diagnostic and therapeutic context of nos

• A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the 35S promoter, the nos terminator and the nptII gene [4].

References