Disease relevance of Organisms, Genetically Modified

• The 35S promoter sequence of cauliflower mosaic virus and the terminator of the nopaline synthase (NOS) gene from Agrobacterium tumefaciens were both detected since they are present in most genetically modified organisms [1].

High impact information on Organisms, Genetically Modified

• To analyze these findings, we isolated cDNAs in and around the misato locus, mapped the breakpoints of chromosomal deficiencies, determined which transcript corresponded to the misato gene, rescued the cell division defects in transgenic organisms, and sequenced the genomic DNA [2].
• However, these calmodulin-based types of probes have a series of deficiencies, such as reduced dynamic ranges, when expressed within transgenic organisms and lack of calcium sensitivity in certain targetings [3].
• This will facilitate establishment of tet regulatory systems under a variety of conditions, but particularly when cell type-restricted tetracycline-controlled gene expression is to be achieved in transgenic organisms via homologous recombination [4].
• Ann Warren and Julian Crampton here discuss how transposable elements can be exploited as valuable research tools for the molecular characterization of genomes and as DNA vectors for genome manipulation and the 'creation' of transgenic organisms [5].
• Coupled with electronic devices for visualizing and quantifying FPs, recently cloned FP genes might be useful for the ecological monitoring of transgenic organisms in the environment [6].

Biological context of Organisms, Genetically Modified

• This trans-dominant negative phenotype, seen when the CAG repeat yields a poly-Ala stretch, may turn out to be an excellent tool for functional analysis of GR in transgenic organisms [7].

Associations of Organisms, Genetically Modified with chemical compounds

• Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea [8].
• PCR methods for the detection of genetically modified organisms (GMOs) were developed that can be used for screening purposes and for specific detection of glyphosate-tolerant soybean and insect-resistant maize in food [9].
• Luciferases are enzymes that emit light in the presence of oxygen and a substrate (luciferin) and which have been used for real-time, low-light imaging of gene expression in cell cultures, individual cells, whole organisms, and transgenic organisms [10].

Gene context of Organisms, Genetically Modified

• We are testing these predictions directly by using biophysical and biochemical techniques to characterize myosin isolated from transgenic organisms [11].
• In recent years, the development of transgenic organisms has allowed a more complete understanding of syndecan function [12].
• High-throughput double quantitative competitive polymerase chain reaction for determination of genetically modified organisms [13].
• The technologies that enable production of knockout mice, Arabidopsis mutants and overexpression in transgenic organisms have revealed the contribution of FKBP to development in higher eukaryotes [14].
• A highly sensitive quantitative real-time assay targeted on the 35S promoter of a commercial genetically modified organism (GMO) was characterized (sF/sR primers) and developed for an ABI Prism 7700 Sequence Detection System and TaqMan chemistry [15].

Analytical, diagnostic and therapeutic context of Organisms, Genetically Modified

• Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods [16].

References