High impact information on TMEM123

• Anti-Porimin (Pro-oncosis receptor inducing membrane injury) mAb mediates oncosis-like cell death in Jurkat cells [1].
• Porimin cDNA was isolated from a Jurkat cell cDNA library by COS cell-expression cloning [1].
• The Porimin gene was mapped to human chromosome 11q22.1 and is composed of four exons spanning 133 kb of genomic DNA [1].
• The extracellular domain of Porimin contains many O-linked and seven N-linked glycosylation sites that define it as a new member of the mucin family [1].
• Crosslinking of the Porimin receptor expressed on COS7 transfectants resulted in the loss of cell membrane integrity and cell death as measured by the leakage of intracellular lactate dehydrogenase [1].

Biological context of TMEM123

• Molecular cloning of Porimin, a novel cell surface receptor mediating oncotic cell death [1].
• Both COS7 and 293 cells expressing transfected Porimin at a relatively high level lost their ability to adhere to culture dishes, suggesting a role for Porimin in cell adhesion [1].

Other interactions of TMEM123

• When expressed in Jurkat cells, a His-tagged Porimin cDNA construct resulted in the generation of a specific 110-kDa-size protein that matched the molecular mass of the endogenous Porimin protein [1].

Analytical, diagnostic and therapeutic context of TMEM123

• COS7 and 293 cells transiently transfected with Porimin cDNA were specifically recognized by anti-Porimin Ab in cell staining and immunoblotting experiments [1].

References