Disease relevance of NOSTRIN

• OBJECTIVE: To investigate the expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) in the placenta of women with pre-eclampsia (PE) and discuss its role in the pathogenesis of PE [1].

High impact information on NOSTRIN

• NOSTRIN colocalized extensively with eNOS at the plasma membrane of confluent human umbilical venous endothelial cells and in punctate cytosolic structures of CHO-eNOS cells [2].
• NOSTRIN overexpression induced a profound redistribution of eNOS from the plasma membrane to vesicle-like structures matching the NOSTRIN pattern and at the same time led to a significant inhibition of NO release [2].
• NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells [2].
• Here, we show that NOSTRIN directly binds to caveolin-1, a well-established inhibitor of eNOS [3].
• Similarly, when N-WASP-GFP and NOSTRIN are co-expressed in CHO-eNOS cells, both proteins strongly co-localize with eNOS and are recruited to structures running along actin filaments [4].

Biological context of NOSTRIN

• Sequence similarity of NOSTRIN and pacsins/syndapins suggested a role for NOSTRIN in endocytosis [4].
• NOSTRIN, an NO synthase binding protein, belongs to the PCH family of proteins, exposing a typical domain structure [5].

Anatomical context of NOSTRIN

• If, however, the actin cytoskeleton is depolymerized by cytochalasin D, NOSTRIN and eNOS are associated with extended structures in the cell periphery, possibly being unable to leave the plasma membrane [4].

Associations of NOSTRIN with chemical compounds

• Accordingly, trafficking of NOSTRIN/eNOS/caveolin is affected by altering the state of actin filaments or cholesterol levels in the plasma membrane [3].

Other interactions of NOSTRIN

• NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS [4].
• Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN [3].

Analytical, diagnostic and therapeutic context of NOSTRIN

• METHODS: Western blot analysis was used to detect the expression of NOSTRIN and endothelial nitric oxide synthase (eNOS) [1].
• RESULTS: Western blot analysis showed that the protein level of NOSTRIN was significantly higher in women with PE than in the control group (P<0.01) [1].

References