Disease relevance of int

• Molecular organization in site-specific recombination: the catalytic domain of bacteriophage HP1 integrase at 2.7 A resolution [1].
• HP1 integrase promotes site-specific recombination of the HP1 genome into that of Haemophilus influenzae [1].
• The deduced amino acid sequence of HP1 integrase has remarkable similarity to that deduced for the integrase of coliphage 186 [2].
• The dimerization deficient mutants were unaffected in binding to its phage attachment site (attP) substrate, but had a reduced ability to complement an int-defective prophage [3].
• In addition to the P2 integrase, the accessory proteins Escherichia coli IHF and P2 Cox are needed for recombination [4].

High impact information on int

• Binding sites for bacteriophage HP1 integrase on its DNA substrates [5].
• At sites of type I, integrase binding almost completely eliminated cleavage by DNase I; type I sites shared the consensus sequence 5'-AGGGATTTWW [5].
• Integrase-mediated excision occurs via recombination between a 22 bp sequence at the 3' terminus of pheV and an imperfect direct repeat at the pheV-distal boundary of the PAI [6].
• P2 integrase belongs to the tyrosine family of recombinases [3].
• Int belongs to the large family of tyrosine recombinases that have two different DNA recognition motifs binding to the arm and core sites, respectively, which are located within the phage attachment sites (attP) [4].

Biological context of int

• This sequence contained an open reading frame encoding a 337-residue polypeptide, which is a member of the integrase family of site-specific recombination proteins as judged by sequence comparison [2].
• Coliphage P2 integrates into the host chromosome upon lysogenization via site-specific recombination mediated by the phage integrase (Int) [3].
• Bacteriophage P2 integrase (Int) mediates site-specific recombination leading to integration or excision of the phage genome in or out of the bacterial chromosome [4].

References