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Gene Review:

int - Int

Enterobacteria phage P22

Lindsey, D.F. et al., Casavant, N.C. et al., Mavris, M. et al., Smith-Mungo, L. et al.

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Disease relevance of int

Characterization of the cryptic lambdoid prophage DLP12 of Escherichia coli and overlap of the DLP12 integrase gene with the tRNA gene argU [1].
The argU and DLP12 int genes overlapped at their 3' ends, and argU contained sequence homologous to a portion of the phage P22 attP site [1].

High impact information on int

The bacterial DNA target, attB, is approximately 27 base pairs and consists of two core type Int binding sites as inverted repeats [2].
These genes are adjacent to the integrase gene (int) and attachment site (attP), which are highly homologous to those of Salmonella bacteriophage P22 [3].
The presence of bioavailable arabinose triggered the production of P22 excisionase and integrase from the reporter plasmid pAraLHB in JL1157, and this led to excision of the cI repressor gene, which is flanked by att sites, and the subsequent irreversible expression of gfp in the original cell and in its progeny [4].

References

Characterization of the cryptic lambdoid prophage DLP12 of Escherichia coli and overlap of the DLP12 integrase gene with the tRNA gene argU. Lindsey, D.F., Mullin, D.A., Walker, J.R. J. Bacteriol. (1989) [Pubmed]
Structure of the P22 att site. Conservation and divergence in the lambda motif of recombinogenic complexes. Smith-Mungo, L., Chan, I.T., Landy, A. J. Biol. Chem. (1994) [Pubmed]
Mechanism of bacteriophage SfII-mediated serotype conversion in Shigella flexneri. Mavris, M., Manning, P.A., Morona, R. Mol. Microbiol. (1997) [Pubmed]
Site-specific recombination-based genetic system for reporting transient or low-level gene expression. Casavant, N.C., Beattie, G.A., Phillips, G.J., Halverson, L.J. Appl. Environ. Microbiol. (2002) [Pubmed]

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