Disease relevance of int

• The argU and DLP12 int genes overlapped at their 3' ends, and argU contained sequence homologous to a portion of the phage P22 attP site [1].
• This organization of the DLP12 sequences and the presence of the argU/DLP12 int pair in several E. coli strains and closely related species suggest that DLP12 might be an ancestral lambdoid prophage [1].
• Bacteriophage lambda int gene is required for the integration of viral DNA into the chromosome of Escherichia coli [2].
• Regulation of integration by coliphage lambda: activation of int transcription by the cII and cIII proteins [3].

High impact information on int

• Sequence analyses of the mutations reveal single base changes, spanning 37 base pairs (bp) in the b region, some 240 bp beyond the int gene [4].
• The bacteriophage lambda int gene product, integrase, recombines the phage DNA with the host DNA at specific sites on each to accomplish lysogeny [4].
• Modification of int with N-ethylmaleimide selectively alters its interaction with arm-type sequences [5].
• Because RNase III host mutants are defective in sib regulation, processing of the PL mRNA at sib by this endoribonuclease may cause int mRNA decay and decrease int synthesis [4].
• The bacteriophage lambda int gene product. A filter assay for genetic recombination, purification of int, and specific binding to DNA [2].

Biological context of int

• The structures show how the simultaneous binding of two separate domains of lambda-int to DNA facilitates synapsis and can specify the order of DNA strand cleavage and exchange [6].
• After transduction of each copy, a helper plasmid bearing phage lambda xis and int genes is introduced into the cells to excise the drug resistance gene flanked with the lambda attL and lambda attR sites from the chromosome [7].
• The DNA sequence of the int and xis genes of lambda bacteriophage, as well as that of the PI promoter and a region of unknown function beyond this, was determined by the chain termination procedure [8].
• To analyse the mechanism by which rare codons near the initiation codon inhibit cell growth and protein synthesis, we used the bacteriophage lambda int gene or early codon substitution derivatives [9].
• Removal of a terminator structure by RNA processing regulates int gene expression [10].

Other interactions of int

• The cII-independent expression of the phage lambda int gene in RNase III-defective E. coli [11].

References