Disease relevance of traY

• lambda-Transducing phages carrying transfer genes isolated from an abnormal prophage insertion into the traY gene of F [1].
• Characterization of the Escherichia coli F factor traY gene product and its binding sites [2].

High impact information on traY

• Deletion in the amino-terminal part of the traI polypeptide abolishes the oriT-nicking activity while leaving the strand-separating activity intact [3].
• The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon [4].
• The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT [5].
• The traJ gene product is the positive regulator of transcription of the 30 kb tra operon, the first gene of which is traY [6].
• Regulation of the F plasmid traY promoter in Escherichia coli by host and plasmid factors [7].

Biological context of traY

• The sequences of the traJ gene and the 5' end of the traY gene of the resistance plasmid R1 [8].
• By primer extension analysis, transcription initiation sites for traY mRNA synthesis were unaltered by the mutations [9].
• By sequence analysis, all seven proved to be IS1 or IS2 insertions defining five insertion sites between base-pairs -49 and -82 with respect to the major traY transcription initiation site [9].
• Sfr function was measured by activation of a plasmid traY-lacZ gene fusion [10].

Associations of traY with chemical compounds

• We have located the traY leads to Z operon promoter by in vitro run-off transcription experiments and have developed in vivo assays for the activity of the promoter by fusing it to galactokinase and kanamycin-resistance genes [11].

Enzymatic interactions of traY

• Addition of TraYp to nicking reactions catalyzed by helicase I showed no effect on the rate or efficiency of oriT nicking [2].

Other interactions of traY

• Genetic studies have shown nick formation to be dependent on both the traI and traY genes [5].
• Transcription of the transfer genes traY and traM of the antibiotic resistance plasmid R100-1 is linked [12].

Analytical, diagnostic and therapeutic context of traY

• A sequencing stop after nucleotide 137 of this strand (where oriT-nicking seems to occur in vivo) is possibly an artefact caused by helicase I protein attached to the 5' terminal nucleotide [3].

References