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Gene Review

UL82  -  upper matrix protein; involved in gene...

Human herpesvirus 5

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Disease relevance of HHV5gp077

  • Even though the major immediate-early promoter of human cytomegalovirus is a strong enhancer-containing promoter, UL82 further enhanced its transcription as much as 20-fold [1].
  • UL82 virion protein activates expression of immediate early viral genes in human cytomegalovirus-infected cells [2].
  • MHC molecules were analyzed in glioblastoma cells exposed to a replication-defective adenovirus expressing UL82 (Adpp71) or after transient transfection of the UL82 gene [3].

High impact information on HHV5gp077

  • It was propagated on human diploid fibroblasts expressing the UL82 gene product, and it was possible to produce a mutant virus lacking the UL82 protein by passaging virus stocks for one cycle of growth on normal, noncomplementing fibroblasts [2].
  • The growth phenotype associated with the UL82 mutant seems to result from its inability to efficiently activate human cytomegalovirus immediate early genes [2].
  • The UL82 gene product is delivered to the nucleus at the time of infection, and it is believed to function in gene activation [2].
  • In addition, we demonstrate that the impaired growth phenotype associated with the UL82 (pp71) deletion mutant is abolished when hDaxx knockdown cells are infected, suggesting that pp71 functions to relieve hDaxx-mediated repression during HCMV infection [4].
  • UL82 expression did not interfere with accumulation of either MHC class I heavy-chain transcript or protein, nor did UL82 expression correlate with markers of apoptosis [3].

Biological context of HHV5gp077

  • The human cytomegalovirus UL82-encoded pp71 protein is required for efficient virus replication and immediate-early gene expression when cells are infected at a low multiplicity [5].
  • Mutation in the carboxy-terminal region of UL82 also eliminated transactivation [1].
  • The transcript could be involved in the posttranscriptional regulation of the UL82 gene, affecting its mRNA stability or translation [6].
  • The antibody was found to react with a protein homologous to the human cytomegalovirus (HCMV) matrix protein (pp71), the product of the UL82 open reading frame (ORF). mAbs were generated from heterologous fusion of spleen cells from PCMV-positive mice and Balb/C P3X63-Ag8.653 myeloma cells [7].

Anatomical context of HHV5gp077

  • Using UL82 mutant viruses, we demonstrate that the LXCXD motif within pp71 is not necessary for efficient virus replication in fibroblasts, suggesting that pp71's ability to degrade hypophosphorylated Rb family members and induce quiescent cells into the cell cycle is not responsible for the growth defect associated with a UL82 deletion mutant [5].
  • This contradiction is discussed in light of the fact that the 4-kb mRNA, which codes for pp65 and was targeted in UL83-antisense cell lines, may be a bicistronic mRNA which also codes for pp71 (UL82) [8].


  1. Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cis-acting elements. Liu, B., Stinski, M.F. J. Virol. (1992) [Pubmed]
  2. UL82 virion protein activates expression of immediate early viral genes in human cytomegalovirus-infected cells. Bresnahan, W.A., Shenk, T.E. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  3. Human cytomegalovirus protein pp71 disrupts major histocompatibility complex class I cell surface expression. Trgovcich, J., Cebulla, C., Zimmerman, P., Sedmak, D.D. J. Virol. (2006) [Pubmed]
  4. Human cytomegalovirus (HCMV) UL82 gene product (pp71) relieves hDaxx-mediated repression of HCMV replication. Cantrell, S.R., Bresnahan, W.A. J. Virol. (2006) [Pubmed]
  5. Interaction between the human cytomegalovirus UL82 gene product (pp71) and hDaxx regulates immediate-early gene expression and viral replication. Cantrell, S.R., Bresnahan, W.A. J. Virol. (2005) [Pubmed]
  6. Characterization of an antisense transcript spanning the UL81-82 locus of human cytomegalovirus. Bego, M., Maciejewski, J., Khaiboullina, S., Pari, G., St Jeor, S. J. Virol. (2005) [Pubmed]
  7. Identification of a monoclonal antibody from Peromyscus maniculatus (deer mouse) cytomegalovirus (PCMV) which binds to a protein with homology to the human CMV matrix protein HCMV pp71. Deyde, V., Rizvanov, A., Otteson, E., Brandt, S., Bego, M., Pari, G., Kozel, T., St Jeor, S. J. Virol. Methods (2005) [Pubmed]
  8. Stably expressed antisense RNA to cytomegalovirus UL83 inhibits viral replication. Dal Monte, P., Bessia, C., Ripalti, A., Landini, M.P., Topilko, A., Plachter, B., Virelizier, J.L., Michelson, S. J. Virol. (1996) [Pubmed]
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