High impact information on Ldb2

• By contrast, CLP-36 mRNA is expressed in several nonmuscle tissues, and here we demonstrate high expression of CLP-36 in epithelial cells by in situ hybridization analysis [1].
• Our subcellular localization studies indicate that in nonmuscle cells, CLP-36 protein localizes to actin stress fibers [1].
• Transcription factor activity of the LIM-HD proteins requires the formation of a tetrameric complex consisting of two LIM-HD molecules linked by a dimer of cofactor (Clim) molecules [2].
• Significantly, in some cases, Lmo or Clim gene expression is robust where no LIM-HD gene expression is detectable [2].
• Ldb2 is ubiquitous at gastrulation, and later is found in many but not all tissues, especially the anterior central nervous system (CNS) and vasculature [3].

Biological context of Ldb2

• However, little is known about the nature of CLIM1b or the evolutionary conservation of this type of alternative splicing in amphibians and teleost fish [4].
• Human CLIM1 and CLIM2 genes were mapped to the chromosome on 4p15.3 and 10q24-q25 regions, respectively [5].

Anatomical context of Ldb2

• Here we report the isolation of human CLIM1 and CLIM2 cDNAs and the determination of their chromosome locations by using a human-rodent monochromosomal hybrid cell panel and a radiation hybrid mapping panel [5].

Analytical, diagnostic and therapeutic context of Ldb2

• Interestingly, immunoprecipitation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis indicate that both nonmuscle alpha-actinin-1 and alpha-actinin-4 form complexes with CLP-36 [1].

References