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Gene Review:

tbg-1 - Protein TBG-1

Caenorhabditis elegans

Strome, S. et al., Hannak, E. et al., Hannak, E. et al., McNally, K. et al., Ren, X. et al., et al.

Strome, Powers, Dunn, Reese, Malone, White, Seydoux, Saxton,

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High impact information on tbg-1

In addition, double-mutant analysis indicated that gamma-tubulin-dependent nucleation and microtubule severing may provide redundant mechanisms for increasing microtubule number during the early stages of meiotic spindle assembly [1].
By kinetically monitoring MT regrowth from cold-treated mitotic centrosomes in vivo, we show that centrosomal nucleating activity is severely compromised by gamma-tubulin depletion [2].
Centrosomes mature as cells enter mitosis, accumulating gamma-tubulin and other pericentriolar material (PCM) components [3].
Our results suggest that gamma-tubulin is not absolutely required for microtubule nucleation in C. elegans but is required for the normal organization and function of kinetochore and interpolar microtubules [4].
We find that the mitotic spindle is diastral and symmetric through early metaphase, then becomes asymmetric following the transient down-regulation of one centrosome, as judged by gamma-tubulin immunofluorescence [5].

Biological context of tbg-1

To study the role of gamma-tubulin in nucleation and organization of spindle microtubules, we used RNA interference (RNAi) to deplete C. elegans embryos of gamma-tubulin. gamma-Tubulin (RNAi) embryos failed in chromosome segregation, but surprisingly, they contained extensive microtubule arrays [4].
Consistent with this hypothesis, we find that AIR-1 is required for the increase in centrosomal gamma-tubulin and two other PCM components, ZYG-9 and CeGrip, as embryos enter mitosis [3].

Anatomical context of tbg-1

gamma-Tubulin-containing complexes are thought to nucleate and anchor centrosomal microtubules (MTs) [2].

Associations of tbg-1 with chemical compounds

SPD-5 is required for the centrosomal localization of gamma-tubulin, XMAP-215, and Aurora A kinase family members, but SPD-5 accumulates at centrosomes in mutants lacking these proteins [6].

Other interactions of tbg-1

In this article we characterize, from a structural point of view, all 16 members of the tubulin gene family of Caenorhabditis elegans (9 alpha-tubulins, 6 beta-tubulins, and 1 gamma-tubulin) [7].

References

Katanin controls mitotic and meiotic spindle length. McNally, K., Audhya, A., Oegema, K., McNally, F.J. J. Cell Biol. (2006) [Pubmed]
The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent. Hannak, E., Oegema, K., Kirkham, M., Gönczy, P., Habermann, B., Hyman, A.A. J. Cell Biol. (2002) [Pubmed]
Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. Hannak, E., Kirkham, M., Hyman, A.A., Oegema, K. J. Cell Biol. (2001) [Pubmed]
Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans embryos. Strome, S., Powers, J., Dunn, M., Reese, K., Malone, C.J., White, J., Seydoux, G., Saxton, W. Mol. Biol. Cell (2001) [Pubmed]
Asymmetrization of first cleavage by transient disassembly of one spindle pole aster in the leech Helobdella robusta. Ren, X., Weisblat, D.A. Dev. Biol. (2006) [Pubmed]
Centrosome maturation and mitotic spindle assembly in C. elegans require SPD-5, a protein with multiple coiled-coil domains. Hamill, D.R., Severson, A.F., Carter, J.C., Bowerman, B. Dev. Cell (2002) [Pubmed]
Computational prediction of the three-dimensional structures for the Caenorhabditis elegans tubulin family. Gogonea, C.B., Gogonea, V., Ali, Y.M., Merz, K.M., Siddiqui, S.S. J. Mol. Graph. Model. (1999) [Pubmed]

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