Disease relevance of Olfr146

• Cytogenetic characterization of the human prostate cancer cell line P69SV40T and its novel tumorigenic sublines M2182 and M15 [1].
• A rather high expression occurred for M47 and S42 proteins in the Escherichia coli system, whereas for M15 and M17 proteins, a poor expression was observed [2].

High impact information on Olfr146

• The proliferation of D10A cells in response to IL-1 is blocked by M15, but is unaffected by H-7, a potent inhibitor of PKC [3].
• To determine whether both pathways are activated upon occupancy of a single IL-1R, we used a mAb, M15, which blocks binding of IL-1 to the 80-kDa IL-1R, known to be expressed in Th2 cells [3].
• Whereas M15 was able to inhibit the accumulation of cAMP induced by IL-1, it had no effect on the IL-1-induced phosphorylation of the 80-kDa substrate of PKC or the c-fos mRNA expression [3].
• A comparison of the expression profiles of M15 and transfectant C2A was performed using Nylon-based arrays [4].
• Chromosome imbalances resulting from numerical and/or structural abnormalities in the tumor sublines involved several chromosome regions that have previously been implicated in human prostate cancer, such as loss of Xp, Y, 3p (M2182 and M15), 16q (M15), and gains for 5q (M2182) and 8q (M2182 and M15) [1].

Biological context of Olfr146

• Segments of chromosomes 3, 15 and X could not be identified even in rearranged chromosomes (Ml to M15) [5].
• They opsonised group-A type M-15 streptococci for phagocytosis by human granulocytes in the presence of fresh human serum [6].

Anatomical context of Olfr146

• Cytogenetic studies of a SV40T antigen immortalized human prostate epithelial cell line (P69SV40T) and its increasingly tumorigenic tumor sublines, designated M2182 and M15, were done with GTG-banding and multicolor fluorescence in situ hybridization (FISH) [1].

Analytical, diagnostic and therapeutic context of Olfr146

• Cells were harvested just after the finish of the 24 h stimulation treatment or 24 h later, and three mitochondrial fractions were isolated by differential centrifugation: the M1 fraction (1000 g), the M3 fraction (3000 g) and the M15 fraction (15,000 g) [7].

References