High impact information on GGTLA1

• The genes in the repeat are GGT, BCRL, V7-rel, POM121-like, and GGT-rel [1].
• The deduced amino acid sequence of this cDNA, GGT-rel, exhibited an overall similarity of 39.5% with human GGT [2].
• Experiments with glutathione added to the medium suggested that GGT-rel could hydrolyze the gamma-glutamyl moiety [2].
• The present study demonstrates for the first time that an established cell line expressing GGT-rel, a human GGT-related enzyme, metabolizes extracellular GSH to cysteinylglycine (CysGly) in a time-dependent manner when cells were incubated in a medium containing 2.5 mM GSH and 25 mM glycylglycine [3].
• A higher iron reduction and an increased LPO level determined by malondialdehyde HPLC measurement were also found in GGT-rel-overexpressing cells compared to GGT-rel negative cells [3].

Biological context of GGTLA1

• The chromosomal location of this related gene, GGTLA1, has been determined by both isotopic and fluorescence in situ hybridization to metaphase cells and by Southern blot analysis of somatic cell hybrid DNAs [4].

Other interactions of GGTLA1

• Some of the GGT and GGTLA family members are located on NotI restriction enzyme fragments of a similar size [4].

References