High impact information on Cenph

• We examined the role of CENP-H in centromere function and assembly by generating a conditional loss-of-function mutant in the chicken DT40 cell line [1].
• In the absence of CENP-H, cell cycle arrest at metaphase, consistent with loss of centromere function, was observed [1].
• These results suggest that CENP-H multimers localize constitutively to the inner kinetochore plate and play an important fundamental role in organization and function of the active human centromere-kinetochore complex [2].
• Furthermore, CENP-H was detected at neocentromeres, but not at inactive centromeres in stable dicentric chromosomes [2].
• Immunofluorescence stainings of mouse fibroblast cells with anti-CENP-H-specific antibody demonstrated that the CENP-H is specifically and constitutively localized in kinetochores throughout the cell cycle; this was also confirmed by stainings with anti-centromere-specific antibody [3].

Biological context of Cenph

• Assignment of the centromere protein H (Cenph) gene to mouse chromosome band 13D1 by in situ hybridization and interspecific backcross analyses [4].
• Characterization of a novel kinetochore protein, CENP-H [3].

References