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Gene Review:

pol - pol

Feline foamy virus

Holzschu, D.L. et al., Heinkelein, M. et al., Flügel, R.M. et al., Enssle, J. et al., Li, H. et al., et al.

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Disease relevance of pol

The location of the splice acceptor of the human foamy virus pol mRNA was confirmed to map in gag [1].
In contrast to other known retroviruses, the FV pol genes are expressed via spliced transcripts [2].
The incorporation of nonvector RNA into vector particles could be reduced to barely detectable levels with constructs in which the human immunodeficiency virus 5' SD or an unrelated intron sequence was substituted for the FV 5' untranslated region and in which gag expression and pol expression were separated on two different plasmids [3].
We used the polymerase chain reaction to attempt to amplify viral DNA from tumour tissues, using primers from the pol and tax (HTLV-I) and gag and bel1 (HFV) regions [4].

High impact information on pol

Downstream of a small intergenic pol-env region a long open reading frame of 985 amino acid residues was identified that according to its genomic location, size, glycosylation signals, and hydrophobicity profile closely resembles the lentiviral env genes [5].
In the foamy virus (FV) subgroup of retroviruses the pol genes are located in the +1 reading frame relative to the gag genes and possess potential ATG initiation codons in their 5' regions [6].
The nucleotide sequence and spliced pol mRNA levels of the nonprimate spumavirus bovine foamy virus [7].

Biological context of pol

We have determined the complete nucleotide sequence of a replication-competent clone of bovine foamy virus (BFV) and have quantitated the amount of splice pol mRNA processed early in infection [7].

Analytical, diagnostic and therapeutic context of pol

As with the human foamy virus (HFV) and feline foamy virus, we have detected a spliced pol mRNA by PCR [7].

References

Characterization of the spliced pol transcript of feline foamy virus: the splice acceptor site of the pol transcript is located in gag of foamy viruses. Bodem, J., Löchelt, M., Winkler, I., Flower, R.P., Delius, H., Flügel, R.M. J. Virol. (1996) [Pubmed]
Detection of subgenomic cDNAs and mapping of feline foamy virus mRNAs reveals complex patterns of transcription. Bodem, J., Löchelt, M., Delius, H., Flügel, R.M. Virology (1998) [Pubmed]
Improved primate foamy virus vectors and packaging constructs. Heinkelein, M., Dressler, M., Jármy, G., Rammling, M., Imrich, H., Thurow, J., Lindemann, D., Rethwilm, A. J. Virol. (2002) [Pubmed]
Absence of human T-cell lymphotropic virus type I and human foamy virus in thymoma. Li, H., Loehrer, P.J., Hisada, M., Henley, J., Whitby, D., Engels, E.A. Br. J. Cancer (2004) [Pubmed]
Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes. Flügel, R.M., Rethwilm, A., Maurer, B., Darai, G. EMBO J. (1987) [Pubmed]
Foamy virus reverse transcriptase is expressed independently from the Gag protein. Enssle, J., Jordan, I., Mauer, B., Rethwilm, A. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
The nucleotide sequence and spliced pol mRNA levels of the nonprimate spumavirus bovine foamy virus. Holzschu, D.L., Delaney, M.A., Renshaw, R.W., Casey, J.W. J. Virol. (1998) [Pubmed]

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