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Gene Review:

IL12B - interleukin 12B

Bos taurus

Pyeon, D. et al., Premraj, A. et al., Premraj, A. et al., Haas, K.M. et al.

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Disease relevance of IL12B

In contrast, IL-12 p40 mRNA expression by cells from animals with late-stage disease, termed persistent lymphocytosis, was significantly decreased compared to that by normal and alymphocytotic animals [1].
Here, by using quantitative competitive PCR, we show that peripheral blood mononuclear cells from bovine leukemia virus-infected animals in the alymphocytotic stage of disease express an increased amount of IL-12 p40 mRNA [1].
Buffalo IL-12 p40 recombinant protein expressed in Escherichia coli cross-reacted with cattle anti-IL-12 p40 monoclonal antibody [2].

High impact information on IL12B

IL-12 p40 expression occurred only in monocytes/macrophages, not B or T lymphocytes [1].
IL-12 p40 transcripts were produced by B cells activated by CD40, but not by BCR, ligation [3].

Analytical, diagnostic and therapeutic context of IL12B

Culture supernatants from transfected cells contained a hetero-dimeric p70 subunit as revealed in western blot of the proteins separated by native polyacrylamide gel electrophoresis (PAGE) using a monoclonal antibody against bovine IL-12 p40 [4].

References

Interleukin-12 p40 mRNA expression in bovine leukemia virus-infected animals: increase in alymphocytosis but decrease in persistent lymphocytosis. Pyeon, D., Splitter, G.A. J. Virol. (1998) [Pubmed]
Interleukin-12 subunits p35 and p40 of Indian water buffalo (Bubalus bubalis) maintain high sequence homology with those of other ruminants. Premraj, A., Sreekumar, E., Nautiyal, B., Rasool, T.J. International journal of immunogenetics. (2005) [Pubmed]
Activation of bovine B cells via surface immunoglobulin M cross-linking or CD40 ligation results in different B-cell phenotypes. Haas, K.M., Estes, D.M. Immunology (2000) [Pubmed]
Buffalo (Bubalus bubalis) interleukin-12: analysis of expression profiles and functional cross-reactivity with bovine system. Premraj, A., Sreekumar, E., Jain, M., Rasool, T.J. Mol. Immunol. (2006) [Pubmed]

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IL12B	Canis lupus familiaris
il12bb	Danio rerio
il12ba	Danio rerio
IL12B	Gallus gallus
IL12B	Homo sapiens
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Il12b	Rattus norvegicus

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