High impact information on TIMAP

• The frequency of the TIMAP SAGE tag is much greater in endothelial cell SAGE databases than in nonendothelial cells [1].
• Immunofluorescence studies of rat tissues show that anti-TIMAP antibodies localize to vascular endothelium [1].
• Representational difference analysis of the glomerular endothelial cell response to transforming growth factor-beta1 (TGF-beta1) revealed a novel gene, TIMAP (TGF-beta-inhibited membrane-associated protein), which contains 10 exons and maps to human chromosome 20.q11.22 [1].
• The protein phosphatase-1 targeting subunit TIMAP regulates LAMR1 phosphorylation [2].
• TIMAP amino acids 261-290, representing the fourth ankyrin repeat of TIMAP, are necessary and sufficient for the interaction [2].

Anatomical context of TIMAP

• In endothelial cells, endogenous TIMAP and LAMR1 co-immunoprecipitated and co-localized at the plasma membrane [2].

Physical interactions of TIMAP

• We found that TIMAP interacts with the 37/67 kDa laminin receptor (LAMR1) in yeast two-hybrid assays [2].

Other interactions of TIMAP

• In MDCK cells, PP-1 associated with LAMR1 in the presence, but not in the absence, of TIMAP [2].

Analytical, diagnostic and therapeutic context of TIMAP

• By Northern blot, TIMAP mRNA is highly expressed in all cultured endothelial and hematopoietic cells [1].

References