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Gene Review:

IGKV3D-25 - immunoglobulin kappa variable 3D-25...

Homo sapiens

Synonyms: A6, IGKV3D25

Dan, M.D. et al., Vujanovic, L. et al., Sogabe, S. et al., Ruegg, N. et al., Straubinger, B. et al.

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Disease relevance of IGKV3D-25

The recognition of a glioma-associated, cell cycle-independent surface antigen by MAb BT32/A6 makes it a promising candidate for further studies aimed at elucidating its usefulness as an adjunct in the treatment of human malignant gliomas [1].
Thus, using recombinant native-like proteins made in E. coli, the ten N-terminal residues of the receptor were found not to be essential for domain folding, nor for recognition by the antibodies A6, D2 and gamma R38 [2].

High impact information on IGKV3D-25

The two genes which are called A6 and A22 are located in duplicated gene clusters [3].
Neutralizing epitopes on the extracellular interferon gamma receptor (IFNgammaR) alpha-chain characterized by homolog scanning mutagenesis and X-ray crystal structure of the A6 fab-IFNgammaR1-108 complex [4].
Dendritic cells co-infected with Ad.IL-12 and Ad.IL-18 (DC.IL-12/18) were more effective at stimulating MAGE-A6-specific Th1-type CD4(+) T-cell responses than DCs infected with either of the cytokine vectors alone, control Ad.Psi5 virus or uninfected DCs [5].
Although CD4(+) Type-1T helper (Th1) cells secreting interferon-gamma (IFN-gamma) appear to play an essential role in promoting durable antitumor immunity, we have previously shown that patients with cancer exhibit dysfunctional Th1-type responses against epitopes derived from tumor antigens, such as MAGE-A6 [5].
The purpose of this study was to ascertain how various growth parameters may influence the labeling of SK-MG-1, a human glioma cell line, by BT32/A6, a human immunoglobulin M monoclonal antibody (MAb) [1].

Biological context of IGKV3D-25

The epitopes on the human receptor recognized by three neutralizing antibodies, A6, gammaR38 and gammaR99, have been mapped by homolog scanning mutagenesis [4].
Labeling of SK-MG-1 cells by BT32/A6, however, was shown to be unaffected by culture split ratio (p > 0.05) and is therefore independent of cell growth rate, culture viability, and cell cycle state [1].

Analytical, diagnostic and therapeutic context of IGKV3D-25

Approximately 100% of SK-MG-1 cells were shown by flow cytometry to express the BT32/A6 antigen [1].

References

Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen. Dan, M.D., Earley, E.M., Griffin, M.C., Maiti, P.K., Prashar, A.K., Yuan, X.Y., Friesen, A.D., Kaplan, H.A. J. Neurosurg. (1995) [Pubmed]
Mutagenesis of immunoglobulin-like domains from the extracellular human interferon-gamma receptor alpha chain and their recognition by neutralizing antibodies monitored by surface plasmon resonance technology. Ruegg, N., Williams, G., Birch, A., Robinson, J.A., Schlatter, D., Huber, W. J. Immunol. Methods (1995) [Pubmed]
Three transposed elements in the intron of a human VK immunoglobulin gene. Straubinger, B., Osterholzer, E., Zachau, H.G. Nucleic Acids Res. (1987) [Pubmed]
Neutralizing epitopes on the extracellular interferon gamma receptor (IFNgammaR) alpha-chain characterized by homolog scanning mutagenesis and X-ray crystal structure of the A6 fab-IFNgammaR1-108 complex. Sogabe, S., Stuart, F., Henke, C., Bridges, A., Williams, G., Birch, A., Winkler, F.K., Robinson, J.A. J. Mol. Biol. (1997) [Pubmed]
IL-12p70 and IL-18 gene-modified dendritic cells loaded with tumor antigen-derived peptides or recombinant protein effectively stimulate specific Type-1 CD4+ T-cell responses from normal donors and melanoma patients in vitro. Vujanovic, L., Ranieri, E., Gambotto, A., Olson, W.C., Kirkwood, J.M., Storkus, W.J. Cancer Gene Ther. (2006) [Pubmed]

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