High impact information on egr2b

• First, vhnf1 promotes r5+r6 identity through an obligate synergy with Fgf signals to activate valentino and krox20 expression [1].
• Widespread overexpression of Pou2 can rescue the segmental krx20 and val domains in spg mutants, but does not induce ectopic expression of these genes [2].
• The latter being established through differential expression of a hierarchy of transcription factors, including Hox genes, Krox20, and Kreisler/Valentino [3].
• Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development [4].
• To begin to examine the function of genes that control early development in the hindbrain, we have screened an embryonic zebrafish cDNA library with a murine krox-20 gene probe that contained the conserved zinc finger regions [4].

Biological context of egr2b

• Immediate early genes krox-24 and krox-20 are rapidly up-regulated after wounding in the embryonic and adult mouse [5].
• Treatments with retinoic acid produced a phenotype similar to that of ectopic hoxb2 expression, including ectopic krx20 (but not valentino) expression in the eye, and fusion of cartilages from pharyngeal arches 1 and 2 [6].
• A novel zinc finger transcription factor resembles krox-20 in structure and in expression pattern in zebrafish [7].

Anatomical context of egr2b

• Furthermore, in the absence of functional rbmx, expression of specific markers for the fore- and hindbrain (otx2, krox20) was severely reduced [8].

Other interactions of egr2b

• The identity of each rhombomere is specified by the expression of conserved transcription factors, including Krox-20, vHnf1, Val (Kreisler, Mafb) and several Hox proteins [9].
• Double in situ hybridization with krox20 and cyp26d1 reveals that cyp26d1 is expressed in presumptive rhombomere 2-4 (r2-r4) at 2-somite stage [10].
• Our results indicate that reduction in Brg1 expression leads to the expansion of the forebrain-specific transcription factor, six3, and marked reduction in expression of the mid/hind-brain boundary and hind-brain genes, engrailed2 and krox20, respectively [11].

References