High impact information on Porin2

• 1) The CG17137 gene may express a porin with a functional role in D. melanogaster [1].
• We have examined the physiological properties of three other Drosophila proteins (CG17137, CG17139, and CG17140) whose primary sequences have significant homology to DVDAC [2].
• Amplification upon germinal and somatic or stage specific mRNA showed that the highest transcription level of Porin 2 is in testis [3].
• Immuno-histochemical studies indicate that Porin 2 is selectively present in spermatozoa tail, where the mitochondria are located, but not in spermatocytes [3].

Biological context of Porin2

• While porin, CG17137, and CG31722-B are expressed to different degrees during embryogenesis, all of these proteins are dramatically reduced relative to cytochrome c content during larvogenesis [4].
• Annotation of the genome sequence of Drosophila melanogaster revealed three genes (CG17137, CG31722-A, and CG31722-B) with homology to porin, the previously described Drosophila VDAC [4].

Anatomical context of Porin2

• The recombinant DmPorin2 protein is able to form channels similar to those formed by DmPorin1 reconstituted in artificial membranes [1].
• Western blot analysis performed with antibodies raised against the recombinant Porin 2 confirmed a high level of expression in the fly spermatozoa [3].

Other interactions of Porin2

• Functional characterization of a second porin isoform in Drosophila melanogaster. DmPorin2 forms voltage-independent cation-selective pores [1].
• Two of these proteins (CG17137 and CG17140) produced channels with anionic selectivity in the open state [2].

References