High impact information on Cyp6g1

• Presence of the insertion is correlated with increased transcript abundance of Cyp6g1, a phenotype previously shown to be associated with insecticide resistance in Drosophila melanogaster [1].
• The 5' flanking sequence of one gene in this region, Cyp6g1 (a cytochrome P450), is nearly fixed for a Doc transposable element insertion [1].
• Our results suggest that more than Cyp6g1 is overtranscribed in field and laboratory DDT-resistant genotypes, and the number of commonalities suggests that similar resistance mechanisms may exist between laboratory- and field-selected DDT-resistant fly lines [2].
• Cyp6g1-mediated insecticide resistance in Drosophila melanogaster is due to the upregulation of the cytochrome P450 gene Cyp6g1, leading to the resistance to a variety of insecticide classes [3].
• Cis-Regulatory Elements in the Accord Retrotransposon Result in Tissue-Specific Expression of the Drosophila melanogaster Insecticide Resistance Gene Cyp6g1 [3].

Biological context of Cyp6g1

• Insecticide resistance in laboratory selected Drosophila strains has been associated with upregulation of a range of different cytochrome P450s, however in recent field isolates of D. melanogaster resistance to DDT and other compounds is conferred by one P450 gene, Cyp6g1 [4].
• Evidence indicates that the gene on chromosome II is Cyp6g1 [5].
• Although the hypothetical selective agent remains unknown, these data are suggestive of a selective advantage brought about by the Bari1 insert and are reminiscent of recent evidence for functional mutagenesis of cyp6g1, another P450 gene, brought about by Accord and Doc transposable elements in D. melanogaster and Drosophila simulans [6].

Anatomical context of Cyp6g1

• We show that the Accord LTR insertion is indeed the resistance-associated mutation and demonstrate that the Accord LTR carries regulatory sequences that increase the expression of Cyp6g1 in tissues important for detoxification, the midgut, Malpighian tubules, and the fat body [3].

Associations of Cyp6g1 with chemical compounds

• Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains of Drosophila melanogaster [7].
• Transgenic flies over-expressing Cyp6g1 also show cross-resistance to other neonicotinoids such as acetamiprid and nitenpyram [4].
• Furthermore, we show that pre-existing Rst(2)DDT alleles in turn confer cross-resistance to imidacloprid [8].

Analytical, diagnostic and therapeutic context of Cyp6g1

• The susceptible phenotype of the Canton-SH and Hikone-RH strains is not due to mutation in the Cyp6g1 gene; sequence analysis showed that Cyp6g1 alleles of resistant and susceptible strains are very similar and cannot be classified into resistant and susceptible alleles [9].

References