Disease relevance of lacG

• The lacG gene encoding the 6-phospho-beta-galactosidase (E.C.3.2.1.85) of Staphylococcus aureus was fused to the protein A gene in the plasmid pRIT2T [1].
• The lacG gene of a 6-phospho-beta-galactosidase negative S. aureus mutant was cloned into E. coli and sequenced [2].
• Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis [3].
• The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined [4].
• We have tested this system with three reporter genes, the 6-phospho-beta-galactosidase gene of Staphylococcus aureus, the luciferase operon of Vibrio harveyi, and the rabbit beta-globin gene; we have found very low basal levels, while, upon T7 infection, transcription is at least as efficient as in other in vivo T7 RNA polymerase systems in use [5].

High impact information on lacG

• Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors [6].
• Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene [4].
• The lacG gene encodes the phospho-beta-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS [4].
• The S. mutans lacE and lacG genes are located in the same operon as the tagatose genes [4].
• A remarkable bias for leucine codons was observed in the lacG genes of these two species [3].

Chemical compound and disease context of lacG

• The Lactococcus lactis subsp. lactis 712 lacG gene encoding phospho-beta-galactosidase was isolated from the lactose mini-plasmid pMG820 and cloned and expressed in Escherichia coli and L. lactis [3].

Biological context of lacG

• The complete nucleotide sequence of the L. lactis lacG gene and its surrounding regions was determined [3].

Analytical, diagnostic and therapeutic context of lacG

• HPLC profiles of V8-protease digestion of native and inhibited protein show significant differences, whereas the inhibited 6-phospho-beta-galactosidase and a point mutant of 6-phospho-beta- galactosidase (E375Q) yield the same proteolytic fragments [6].
• The hybrid protein of protein A and 6-phospho-beta-galactosidase was used as an enzyme conjugate in enzyme-linked immunosorbent assays (ELISA) [1].

References