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Gene Review:

ECH_0170 - variable length PCR target protein

Ehrlichia chaffeensis str. Arkansas

McBride, J.W. et al., Standaert, S.M. et al., Sumner, J.W. et al., Varela, A.S. et al., Paddock, C.D. et al., et al.

McBride, Doyle, Zhang, Cardenas, Popov, Nethery, Woods, Standaert, Yu, Scott, Childs, Paddock, Nicholson, Singleton, Blaser, Yabsley, Little, Sims, Dugan, Stallknecht, Davidson, Paddock, Sumner, Shore, Bartley, Elie, McQuade, Martin, Goldsmith, Childs,

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Disease relevance of ECH_0170

We report herein the identification and molecular characterization of this highly conserved 19-kDa major immunoreactive glycoprotein (gp19) ortholog of the Ehrlichia chaffeensis variable-length PCR target (VLPT) protein [1].

High impact information on ECH_0170

E. canis gp19 has substantial carboxyl-terminal amino acid homology (59%) with E. chaffeensis VLPT and the same chromosomal location; however, the E. chaffeensis VLPT gene (594 bp) has tandem repeats that are not present in the E. canis gp19 gene (414 bp) [1].
Analysis of the 120-kDa OMP gene and variable-length PCR target gene showed size variations resulting from loss or gain of long, direct repeats within the protein coding sequences [2].
The isolates were characterized by molecular analysis of the 16S rRNA gene, the 120-kDa protein gene, and the variable-length PCR target (VLPT) of E. chaffeensis [3].
On the basis of the 120-kDa and VLPT genotypes, the cerebrospinal fluid and blood isolates from the same patients were identical [3].
This is the most extensive study of E. chaffeensis VLPT and 120-kDa antigen gene genetic variation to date and is the first to examine genetic variation in E. chaffeensis from a nonhuman vertebrate host [4].

Biological context of ECH_0170

The isolates were characterized by molecular evaluation of the 16S rRNA gene, the groESL heat shock operon, a 120-kDa immunodominant protein gene, and an incompletely characterized repetitive-motif sequence (variable-length PCR target [VLPT]) [5].

Anatomical context of ECH_0170

PCR amplification of the VLPT gene on whole blood, cell culture, and tissues detected primary and/or secondary strains in all deer exposed to both primary and secondary strains; in one deer, the primary strain was cultured from the lymph node [6].

Analytical, diagnostic and therapeutic context of ECH_0170

This gene, designated the variable-length PCR target, is useful for PCR detection of E. chaffeensis and differentiation of isolates [7].
Molecular cloning and characterization of the Ehrlichia chaffeensis variable-length PCR target: an antigen-expressing gene that exhibits interstrain variation [7].

References

Identification of a Glycosylated Ehrlichia canis 19-Kilodalton Major Immunoreactive Protein with a Species-Specific Serine-Rich Glycopeptide Epitope. McBride, J.W., Doyle, C.K., Zhang, X., Cardenas, A.M., Popov, V.L., Nethery, K.A., Woods, M.E. Infect. Immun. (2007) [Pubmed]
Molecular heterogeneity of Ehrlichia chaffeensis isolates determined by sequence analysis of the 28-kilodalton outer membrane protein genes and other regions of the genome. Cheng, C., Paddock, C.D., Reddy Ganta, R. Infect. Immun. (2003) [Pubmed]
Primary isolation of Ehrlichia chaffeensis from patients with febrile illnesses: clinical and molecular characteristics. Standaert, S.M., Yu, T., Scott, M.A., Childs, J.E., Paddock, C.D., Nicholson, W.L., Singleton, J., Blaser, M.J. J. Infect. Dis. (2000) [Pubmed]
Molecular variation in the variable-length PCR target and 120-kilodalton antigen genes of Ehrlichia chaffeensis from white-tailed deer (Odocoileus virginianus). Yabsley, M.J., Little, S.E., Sims, E.J., Dugan, V.G., Stallknecht, D.E., Davidson, W.R. J. Clin. Microbiol. (2003) [Pubmed]
Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. Paddock, C.D., Sumner, J.W., Shore, G.M., Bartley, D.C., Elie, R.C., McQuade, J.G., Martin, C.R., Goldsmith, C.S., Childs, J.E. J. Clin. Microbiol. (1997) [Pubmed]
Primary and secondary infection with Ehrlichia chaffeensis in white-tailed deer (Odocoileus virginianus). Varela, A.S., Stallknecht, D.E., Yabsley, M.J., Moore, V.A., Howerth, E.W., Davidson, W.R., Little, S.E. Vector Borne Zoonotic Dis. (2005) [Pubmed]
Molecular cloning and characterization of the Ehrlichia chaffeensis variable-length PCR target: an antigen-expressing gene that exhibits interstrain variation. Sumner, J.W., Childs, J.E., Paddock, C.D. J. Clin. Microbiol. (1999) [Pubmed]

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