Disease relevance of mirr

• Using lox/cre-based recombinase-mediated cassette exchange to control integration position, we studied the effect of cHS4 on the silencing of an integrated beta-geo reporter at three genomic sites in K562 erythroleukemia cells [1].
• The cre-loxP site-specific recombination system has been used successfully to remove a marker gene from transgenic yellow fever mosquitoes, Aedes aegypti [2].

High impact information on mirr

• Ectopic expression of mirr in the posterior follicle cells induces a stripe of rhomboid (rho) expression and represses pipe (pip), a gene with a role in the establishment of the dorsal-ventral axis, at a distance [3].
• The Iroquois genes are expressed in the dorsal half of the eye and here we show that they regulate the expression of the secreted molecule Fringe [4].
• mirror, a Drosophila homeobox gene in the Iroquois complex, is required for sensory organ and alula formation [5].
• The excision of the nptII gene conferring resistance to kanamycin has been achieved here using a gene construct based on a heat-inducible cre gene producing a recombinase that eliminates cre and nptII genes flanked by two loxP sites [6].
• Handling three regulatory elements in one transgene: combined use of cre-lox, FLP-FRT, and I-Scel recombination systems [7].

Biological context of mirr

• The cre recombinase was shown to precisely recognize loxP sites in the mosquito genome and catalyze excision [2].

Analytical, diagnostic and therapeutic context of mirr

• Here, we use in vitro site selection to define the DNA-binding preference of the Drosophila Iroquois Mirror. We use electrophoretic mobility shift assays to determine the critical nucleotides for Mirror binding and to show that this site is recognized by other Drosophila Iroquois transcription factors [8].
• Following a heat treatment, second generation regenerants were screened for excision by PCR using nptII, cre, and T-DNA borders primers [6].

References