Disease relevance of HPRT1

• Although chicken myocyte X mouse melanoma heterokaryons showed extinction of muscle specific properties, they continued to synthesize protein and to incorporate [3H]hypoxanthine, presumably due to the continued production of constitutive chicken HPRT [1].

High impact information on HPRT1

• The chicken HPRT gene: a counter selectable marker for the DT40 cell line [2].
• The effect of bromodeoxyuridine on the segregation of the chicken-specific HPRT gene from Chinese hamster-chick red blood cell somatic hybrids [3].
• It was found that the segregation of the chicken-specific HPRT phenotype associated with the loss of a chromosome was potentiated by bromodeoxyuridine administered during the first hour following release of the block [4].
• As a first step towards the development of radiation hybrid maps, we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6,000 rads with HPRT-deficient hamster Wg3hCl2 cells [5].
• On the contrary, in young chickens, the low concentration of final products of purine metabolism, together with a remarkable activity of HPRT and a high concentration of IMP, indicates that metabolic flux converges on the salvage pathway [6].

Anatomical context of HPRT1

• The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines [3].

References