Disease relevance of RecQ5

• A small isoform of the Drosophila RecQ5 protein (RECQ5) has been expressed in Escherichia coli and purified [1].
• The full-length product of RECQ5/QE was expressed in the baculovirus system and was purified [2].
• The Drosophila melanogaster RECQ5/QE gene encodes a member of the DNA helicase family comprising the Escherichia coli RecQ protein and products of the human Bloom's, Werner's, and Rothmund-Thomson syndrome genes [2].

High impact information on RecQ5

• The level of RECQ5/QE, Drosophila melanogaster RecQ5, was increased after the exposure of cultured cells to methyl-methanesulfonate [3].
• DNA-damaging agents and the mei-41 mutation enhanced the phenotype caused by RECQ5/QE overexpression [3].
• Relationships of Drosophila melanogaster RECQ5/QE to cell-cycle progression and DNA damage [3].
• These results suggest that RECQ5/QE interacts with components of the cell-cycle during its progression in response to DNA damage [3].
• The RECQE mRNA accumulates in oocytes and is expressed at high levels in early embryos [4].

Biological context of RecQ5

• The Recqe gene consists of five exons and four introns, and encodes a protein of 1058 amino acids with a predicted molecular weight of 120,000 Da [4].
• Analysis of helicase activity and substrate specificity of Drosophila RECQ5 [5].
• Overexpression of RECQ5/QE perturbed the progression of the cell-cycle in response to DNA damage in the eye imaginal discs [3].

Analytical, diagnostic and therapeutic context of RecQ5

• Gel filtration experiments indicated that RECQ5/QE was present in an oligomeric state [2].

References