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Gene Review

Hel25E  -  Helicase at 25E

Drosophila melanogaster

Synonyms: ATP-dependent RNA helicase WM6, CG7269, DEAD box protein UAP56, Dbp25F, DmRH4, ...
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Disease relevance of Hel25E


High impact information on Hel25E


Biological context of Hel25E


Anatomical context of Hel25E

  • RNA helicase A is an abundant nuclear enzyme of HeLa cells that unwinds double-stranded RNA in a 3' to 5'direction (Lee, C. G., and Hurwitz, J. (1992) J. Biol. Chem. 267, 4398-4407) [1].
  • The Sciara mle gene produces a single transcript, encoding a helicase, which is present in both male and female larvae and adults and in testes and ovaries [13].
  • Our dominant negative analysis of d-mtDNA helicase in cultured cells provides a tractable model for understanding human autosomal dominant progressive external ophthalmoplegia mutations [14].
  • In this study, we found that, in Xenopus pre-stage I and stage I oocytes, nuage and mitochondrial cement contain the spliceosomal Sm proteins, Xcat2 mRNA, and DEAD-box RNA helicase XVLG1 [15].
  • In this study we sought to examine if the producers of blood platelets, megakaryocytic cells (using the cell lines MEG01, DAMI, HEL), expressed these genes [16].

Associations of Hel25E with chemical compounds

  • While the vast majority of mRNAs were unaffected by LMB, the levels of most mRNAs were significantly reduced in cells depleted of NXF1, p15 or UAP56 [6].
  • Mutation of a highly conserved lysine to methionine in helicase domain I eliminated both activities [17].

Regulatory relationships of Hel25E

  • We propose that the HEL protein promotes an open chromatin structure that favors transcription during development by regulating the spread of heterochromatin, and that HEL is regulated by, and may have a role in, the mitotic cell cycle during embryogenesis [12].

Other interactions of Hel25E


Analytical, diagnostic and therapeutic context of Hel25E


  1. Human RNA helicase A is homologous to the maleless protein of Drosophila. Lee, C.G., Hurwitz, J. J. Biol. Chem. (1993) [Pubmed]
  2. Sterility of Drosophila with mutations in the Bloom syndrome gene--complementation by Ku70. Kusano, K., Johnson-Schlitz, D.M., Engels, W.R. Science (2001) [Pubmed]
  3. Nora virus, a persistent virus in Drosophila, defines a new picorna-like virus family. Habayeb, M.S., Ekengren, S.K., Hultmark, D. J. Gen. Virol. (2006) [Pubmed]
  4. The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family. Johnson, K.N., Christian, P.D. J. Gen. Virol. (1998) [Pubmed]
  5. The mle(napts) RNA helicase mutation in drosophila results in a splicing catastrophe of the para Na+ channel transcript in a region of RNA editing. Reenan, R.A., Hanrahan, C.J., Barry, G. Neuron (2000) [Pubmed]
  6. Genome-wide analysis of nuclear mRNA export pathways in Drosophila. Herold, A., Teixeira, L., Izaurralde, E. EMBO J. (2003) [Pubmed]
  7. SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis. Dalmay, T., Horsefield, R., Braunstein, T.H., Baulcombe, D.C. EMBO J. (2001) [Pubmed]
  8. The NTPase/helicase activities of Drosophila maleless, an essential factor in dosage compensation. Lee, C.G., Chang, K.A., Kuroda, M.I., Hurwitz, J. EMBO J. (1997) [Pubmed]
  9. Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Moyer, S.E., Lewis, P.W., Botchan, M.R. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  10. The Saccharomyces cerevisiae Sub2 protein suppresses heterochromatic silencing at telomeres and subtelomeric genes. Lahue, E., Heckathorn, J., Meyer, Z., Smith, J., Wolfe, C. Yeast (2005) [Pubmed]
  11. The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila. Gatfield, D., Le Hir, H., Schmitt, C., Braun, I.C., Köcher, T., Wilm, M., Izaurralde, E. Curr. Biol. (2001) [Pubmed]
  12. A new enhancer of position-effect variegation in Drosophila melanogaster encodes a putative RNA helicase that binds chromosomes and is regulated by the cell cycle. Eberl, D.F., Lorenz, L.J., Melnick, M.B., Sood, V., Lasko, P., Perrimon, N. Genetics (1997) [Pubmed]
  13. Evolution of dosage compensation in Diptera: the gene maleless implements dosage compensation in Drosophila (Brachycera suborder) but its homolog in Sciara (Nematocera suborder) appears to play no role in dosage compensation. Ruiz, M.F., Esteban, M.R., Doñoro, C., Goday, C., Sánchez, L. Genetics (2000) [Pubmed]
  14. Differential phenotypes of active site and human autosomal dominant progressive external ophthalmoplegia mutations in Drosophila mitochondrial DNA helicase expressed in schneider cells. Matsushima, Y., Kaguni, L.S. J. Biol. Chem. (2007) [Pubmed]
  15. Sm proteins, the constituents of the spliceosome, are components of nuage and mitochondrial cement in Xenopus oocytes. Bilinski, S.M., Jaglarz, M.K., Szymanska, B., Etkin, L.D., Kloc, M. Exp. Cell Res. (2004) [Pubmed]
  16. Expression of human TRPC genes in the megakaryocytic cell lines MEG01, DAMI and HEL. Berg, L.P., Shamsher, M.K., El-Daher, S.S., Kakkar, V.V., Authi, K.S. FEBS Lett. (1997) [Pubmed]
  17. A human DNA helicase homologous to the DNA cross-link sensitivity protein Mus308. Marini, F., Wood, R.D. J. Biol. Chem. (2002) [Pubmed]
  18. Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage. Findley, S.D., Tamanaha, M., Clegg, N.J., Ruohola-Baker, H. Development (2003) [Pubmed]
  19. Unusual nucleic acid binding properties of factor 2, an RNA polymerase II transcript release factor. Xie, Z., Price, D.H. J. Biol. Chem. (1998) [Pubmed]
  20. Analysis of helicase activity and substrate specificity of Drosophila RECQ5. Ozsoy, A.Z., Ragonese, H.M., Matson, S.W. Nucleic Acids Res. (2003) [Pubmed]
  21. The Drosophila mus 308 gene product, implicated in tolerance of DNA interstrand crosslinks, is a nuclear protein found in both ovaries and embryos. Pang, M., McConnell, M., Fisher, P.A. DNA Repair (Amst.) (2005) [Pubmed]
  22. Molecular cloning of the gene encoding nuclear DNA helicase II. A bovine homologue of human RNA helicase A and Drosophila Mle protein. Zhang, S., Maacke, H., Grosse, F. J. Biol. Chem. (1995) [Pubmed]
  23. Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells. Hromas, R., Moore, J., Johnston, T., Socha, C., Klemsz, M. Blood (1993) [Pubmed]
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