High impact information on nr6a1

• As a strategy to analyze the role of xGCNF in embryogenesis, we have induced a gain of function by overexpression of full-length (fl) GCNF and a functional inhibition by a dominant-negative (dn) GCNF [1].
• Ectopic posterior overexpression of the full-length xGCNF caused posterior defects and disturbed somite formation [1].
• Anteroposterior patterning and organogenesis of Xenopus laevis require a correct dose of germ cell nuclear factor (xGCNF) [1].
• We have identified an orphan receptor in Xenopus laevis and named it xGCNF based on its high degree of sequence homology to the previously described murine germ cell nuclear factor (mGCNF) [2].
• Transcripts of xGCNF are found in oocytes and in much smaller amounts in the testes [2].

Biological context of nr6a1

• In gel-electrophoresis mobility shift analysis experiments in vitro translated xGCNF and mGCNF proteins both bind specifically as homodimers to the same response element, a direct repeat of the half-site consensus AGGTCA with zero spacing (DRO) [2].

Anatomical context of nr6a1

• Transcription of xGCNF in animal cap explants occurred independent of mesoderm induction [2].

Analytical, diagnostic and therapeutic context of nr6a1

• In developmental Northern blots and RNase protection using RNA from different embryonic stages, zygotic expression of xGCNF peaks at midneurula [2].

References