Disease relevance of entB

• When the entB gene was placed downstream from the strong lambda phage promoter, PR, SEB was synthesized at readily detectable levels in E. coli [1].
• We have cloned the Staphylococcus aureus entB gene in Escherichia coli, using pBR322 as the vector plasmid; however, no detectable staphylococcal enterotoxin B (SEB) was produced by the E. coli clones [1].
• Expression of the entB and entF genes was also examined in an Ent- Shigella fur mutant [2].

High impact information on entB

• Southern hybridization experiments showed that the entB gene was present on identical restriction fragments in the chromosomes of SEB-producer strains [1].
• Maxam-Gilbert sequencing of a part of the entB gene yielded a DNA sequence that corresponds to the known amino acid sequence of SEB [1].
• Plasmids isolated with Tn5 insertions in the 5' half of entB had the phenotype EntB- EntG+ [3].
• The metabolic shift from one diene to the other is caused by a change in activity of isochorismate hydroxymutase and/or isochorismatase which in turn results from growth under iron deficiency or overexpression of genes (entC and/or entB) involved in chrismate metabolism [4].

Chemical compound and disease context of entB

• The Escherichia coli entB gene, coding for the enterobactin biosynthetic enzyme isochorismatase, has been subcloned into the multicopy plasmid pKK223-3 under the control of the tac promoter [5].

Analytical, diagnostic and therapeutic context of entB

• Southern blot data showed a Mu insertion, presumably with polar effects, in the vicinity of the 5' end of entB [3].

References