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Gene Review

entF  -  enterobactin synthase multienzyme complex...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0579, JW0578
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Disease relevance of entF

  • The Escherichia coli entF gene, which encodes the serine-activating enzyme involved in enterobactin synthesis, has been localized to a 4.7-kilobase-pair DNA fragment inserted in the vector pBR328 [1].
  • Whole-cell RNA isolated from E. coli and the Shigella strains was hybridized to 32P-labeled DNA containing the entB gene or a fragment carrying a portion of the entF gene [2].
  • METHODS AND RESULTS: Production of some pediocin-like bacteriocins such as enterocin A in Enterococcus is regulated by a three-component system comprising a histidine kinase (entK ), a response regulator (entR) and an induction factor (entF ) [3].

High impact information on entF

  • A portion of the remaining nucleotide sequence defined a 320-base pair control region for both the fepA and fes-entF messages [4].
  • Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine [5].
  • We have subcloned and overexpressed entF in a multicopy plasmid and attempted to demonstrate L-serine-dependent ATP-[32P]PPi exchange activity and its participation in enterobactin biosynthesis, but the overexpressed enzyme appears to be essentially inactive in crude extract [5].
  • (v) A mutation responsible for the inactivation of the entF gene was mapped to the entF coding region by using entF hybrid gene fusions [6].
  • Expression of entF was only partially derepressed and entB remained fully repressed at all iron concentrations, suggesting that factors other than Fur are responsible for the repression of these enterobactin genes in the Ent- Shigella strains [2].

Chemical compound and disease context of entF


Biological context of entF


Anatomical context of entF

  • Intracellular survival and growth rates of the B. abortus wild-type and entF mutant strains in mouse-derived J774 macrophages were similar, indicating that production of this siderophore was not essential in this model of infection [8].

Associations of entF with chemical compounds

  • This recombinant molecule, pITS32, restored the ability of an entF mutant to grow on low-iron medium and to produce enterobactin [1].
  • The E and F components were shown to catalyze ATP-pyrophosphate exchange reactions dependent on 2,3-dihydroxybenzoate and L-serine, respectively, whereas fractionated extracts of the entE and entF mutant strains lacked these reactions [9].

Other interactions of entF

  • Strains of Escherichia coli K-12 defective in their ability to utilize exogenously supplied iron due to genetic defects in the entF, tonB, fes, or fep gene exhibited elevated levels of the specific outer-membrane receptor for colicin Ia when compared with parental strains [10].
  • The order of the genes was established as entD, fes, entF, fep, and entCAGBE and the nine genes were shown to be distributed across 26 kb of DNA [11].


  1. Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes gene. Pettis, G.S., McIntosh, M.A. J. Bacteriol. (1987) [Pubmed]
  2. Genetics and regulation of enterobactin genes in Shigella flexneri. Schmitt, M.P., Payne, S.M. J. Bacteriol. (1988) [Pubmed]
  3. Potential of the enterocin regulatory system to control expression of heterologous genes in Enterococcus. Hickey, R.M., Twomey, D.P., Ross, R.P., Hill, C. J. Appl. Microbiol. (2003) [Pubmed]
  4. Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter. Pettis, G.S., Brickman, T.J., McIntosh, M.A. J. Biol. Chem. (1988) [Pubmed]
  5. Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine. Rusnak, F., Sakaitani, M., Drueckhammer, D., Reichert, J., Walsh, C.T. Biochemistry (1991) [Pubmed]
  6. Genetic analysis of the enterobactin gene cluster in Shigella flexneri. Schmitt, M.P., Payne, S.M. J. Bacteriol. (1991) [Pubmed]
  7. Ferric enterochelin transport in Yersinia enterocolitica: molecular and evolutionary aspects. Schubert, S., Fischer, D., Heesemann, J. J. Bacteriol. (1999) [Pubmed]
  8. Brucella abortus strain 2308 produces brucebactin, a highly efficient catecholic siderophore. González Carreró, M.I., Sangari, F.J., Agüero, J., García Lobo, J.M. Microbiology (Reading, Engl.) (2002) [Pubmed]
  9. Biosynthesis of enterochelin in Escherichia coli K-12: separation of the polypeptides coded for by the entD, E, F and G genes. Woodrow, G.C., Young, I.G., Gibson, F. Biochim. Biophys. Acta (1979) [Pubmed]
  10. Relationship between the transport of iron and the amount of specific colicin Ia membrane receptors in Escherichia coli. Konisky, J., Soucek, S., Frick, K., Davies, J.K., Hammond, C. J. Bacteriol. (1976) [Pubmed]
  11. Tn5 mutagenesis of the enterochelin gene cluster of Escherichia coli. Laird, A.J., Young, I.G. Gene (1980) [Pubmed]
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