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Gene Review

TFAM  -  mitochondrial transcription factor A

Drosophila melanogaster

Synonyms: CG4217, D-mtTFA, Dmel\CG4217, MTTFA, Tfam, ...
 
 
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High impact information on TFAM

  • RNA interference (RNAi) of d-TFAM by lipofection of haemocyte-derived Kc167 cells with double-stranded RNA reduced d-TFAM to less than 5% of the normal level [1].
  • The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system [2].
  • By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5'-TTATC/G [2].
  • We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene [2].
  • In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus [2].
 

Biological context of TFAM

 

Anatomical context of TFAM

  • D-mtTFA possesses a nuclear-targeting sequence, and is present in the nucleus at the syncytial stage, where bundles of 64 spermatids are present during spermatogenesis [3].
  • In addition, D-mtTFA was detected in brain throughout the developmental process, as well as in non-proliferating tissues, such as flight muscle and cardia, and was also found in spermatids of imagos [3].

References

  1. Drosophila mitochondrial transcription factor A (d-TFAM) is dispensable for the transcription of mitochondrial DNA in Kc167 cells. Goto, A., Matsushima, Y., Kadowaki, T., Kitagawa, Y. Biochem. J. (2001) [Pubmed]
  2. Drosophila mitochondrial transcription factor A: characterization of its cDNA and expression pattern during development. Takata, K., Yoshida, H., Hirose, F., Yamaguchi, M., Kai, M., Oshige, M., Sakimoto, I., Koiwai, O., Sakaguchi, K. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  3. Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development. Takata, K., Inoue, Y.H., Hirose, F., Murakami, S., Shimanouchi, K., Sakimoto, I., Sakaguchi, K. Cell Biol. Int. (2003) [Pubmed]
 
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