High impact information on RpL32

• This result provides direct evidence that the Minute locus at the 99D interval encodes the ribosomal protein 49 [1].
• Multiple mRNAs, transcribed from genes which lie adjacent to a ribosomal protein ( rp49 ) gene, are present during oogenesis, embryogenesis, or both [2].
• The DNA sequences of the ribosomal protein 49 (rp49) region were determined for 34 isochromosomal strains of Drosophila subobscura representing two chromosomal arrangements, the OST and the O3 + 4 gene arrangements, which differ by two overlapping inversions [3].
• Transfer of genetic information in the rp49 region of Drosophila subobscura between different chromosomal gene arrangements [4].
• Nucleotide variation in the region including the ribosomal protein 49 (rp49) gene was investigated by direct sequencing of 10 alleles of Drosophila subobscura from chromosomes differing in gene arrangements [4].

Biological context of RpL32

• Nucleotide sequence analysis in conjunction with primer extension and S1 nuclease protection experiments show that the structure of the rp49 gene consists of a 102 bp 5' exon, a single 59 bp intron, and a 420 bp 3' exon, encoding a total of 132 amino acids [5].
• Molecular and chromosomal phylogeny in the obscura group of Drosophila inferred from sequences of the rp49 gene region [6].
• Given the probable role played by gene conversion in the history of the rp49 region in D. subobscura, estimates of nucleotide diversity within chromosomal class indicate that the O[3 + 4] chromosomal gene arrangement is older than the O[st] arrangement [4].
• DNA variation at the rp49 gene region of Drosophila simulans: evolutionary inferences from an unusual haplotype structure [7].
• Molecular population genetics of the rp49 gene region in different chromosomal inversions of Drosophila subobscura [8].

Anatomical context of RpL32

• In this report we examine the effects of antisense r-protein 49 expression, a gene known to correspond to a Minute mutation An antisense rp49 gene driven by a strong and inducible promoter was transformed into the Drosophila germ line [9].
• Despite the large differences in rp49 mRNA content, the proportion of the rp49 mRNA which was associated with polysomes during oogenesis and early embryogenesis did not differ significantly from the wild type, implying that rp49 protein is overproduced in the transgenic flies [10].

Other interactions of RpL32

• DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene [11].

References