The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

UBC  -  ubiquitin C

Bos taurus

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on UBC

  • These findings support a model in which an Mms2p/Ubc13p complex assembles novel polyubiquitin chains for signaling in DNA repair, and they suggest that UEV proteins may act to increase diversity and selectivity in ubiquitin conjugation [1].
  • Sequence analysis showed them to encode the bovine polyubiquitin (A4), elongation factor 1alpha (B2), the acidic ribosomal phosphoprotein PO (C3), and the ribosomal protein S2 (E10) [2].
  • In vitro studies show that E3alpha forms lysine 48-linked polyubiquitin degradation signals on type 1-3 substrates and is absolutely dependent on Ubc2/Rad6 orthologs [3].
  • Cyclization of polyubiquitin by the E2-25K ubiquitin conjugating enzyme [4].
  • We show that rabbit reticulocyte lysate harbors enzymatic components that catalyze the assembly of unanchored Lys-29-linked polyubiquitin chains [5].
 

Biological context of UBC

  • In this report, we present the results of analyzing the nucleotide sequence of polyubiquitin genes of mammals, available in the DDBJ/EMBL/GenBank nucleotide sequence databases, in which we found that there are two groups of polyubiquitin genes in an orthologous relationship [6].
  • Although the predicted amino acid sequence of the ubiquitin from E. histolytica differs significantly (in 7-9 amino acid residues) from that of yeast and animals, expression of the coding sequence of EhUBI1 suppressed the heat-sensitive phenotype of a polyubiquitin gene-deficient yeast mutant [7].
 

Associations of UBC with chemical compounds

  • E2/E3-mediated assembly of lysine 29-linked polyubiquitin chains [5].
  • Fluorescent labeling of the six cysteines in the ovomucoid moiety (OM) with Lucifer Yellow iodoacetamide yields UbOM(LY); the ubiquitin in the fusion protein can be extended by the addition of a K48-linked polyubiquitin chain to form Ub(n)OM(LY) [8].
 

Enzymatic interactions of UBC

  • The mammalian ubiquitin conjugating enzyme known as E2-25K catalyzes the synthesis of polyubiquitin chains linked exclusively through K48-G76 isopeptide bonds [9].
 

Analytical, diagnostic and therapeutic context of UBC

  • One of these, EhUBI1, was cloned and sequenced and found to correspond to a monoubiquitin gene; as shown by a polymerase chain reaction, E. histolytica lacked polyubiquitin genes altogether [7].

References

  1. Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Hofmann, R.M., Pickart, C.M. Cell (1999) [Pubmed]
  2. Identification of four genes in endothelial cells whose expression is affected by tumor cells and host immune status--a study in ex vivo-isolated endothelial cells. Liliensiek, B., Rocha, M., Umansky, V., Benner, A., Lin, J., Ziegler, R., Nawroth, P.P., Schirrmacher, V. Blood (1998) [Pubmed]
  3. N-end rule specificity within the ubiquitin/proteasome pathway is not an affinity effect. Baboshina, O.V., Crinelli, R., Siepmann, T.J., Haas, A.L. J. Biol. Chem. (2001) [Pubmed]
  4. Cyclization of polyubiquitin by the E2-25K ubiquitin conjugating enzyme. Yao, T., Cohen, R.E. J. Biol. Chem. (2000) [Pubmed]
  5. E2/E3-mediated assembly of lysine 29-linked polyubiquitin chains. Mastrandrea, L.D., You, J., Niles, E.G., Pickart, C.M. J. Biol. Chem. (1999) [Pubmed]
  6. Interspecific comparison in the frequency of concerted evolution at the polyubiquitin gene locus. Nenoi, M., Ichimura, S., Mita, K. J. Mol. Evol. (2000) [Pubmed]
  7. Characterization of ubiquitin genes and -transcripts and demonstration of a ubiquitin-conjugating system in Entamoeba histolytica. Wöstmann, C., Liakopoulos, D., Ciechanover, A., Bakker-Grunwald, T. Mol. Biochem. Parasitol. (1996) [Pubmed]
  8. Ubiquitin-ovomucoid fusion proteins as model substrates for monitoring degradation and deubiquitination by proteasomes. Yao, T., Cohen, R.E. Meth. Enzymol. (2005) [Pubmed]
  9. Core domain mutation (S86Y) selectively inactivates polyubiquitin chain synthesis catalyzed by E2-25K. Mastrandrea, L.D., Kasperek, E.M., Niles, E.G., Pickart, C.M. Biochemistry (1998) [Pubmed]
 
WikiGenes - Universities