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Gene Review

UBC  -  ubiquitin C

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High impact information on UBC

  • These findings support a model in which an Mms2p/Ubc13p complex assembles novel polyubiquitin chains for signaling in DNA repair, and they suggest that UEV proteins may act to increase diversity and selectivity in ubiquitin conjugation [1].
  • Sequence analysis showed them to encode the bovine polyubiquitin (A4), elongation factor 1alpha (B2), the acidic ribosomal phosphoprotein PO (C3), and the ribosomal protein S2 (E10) [2].
  • In vitro studies show that E3alpha forms lysine 48-linked polyubiquitin degradation signals on type 1-3 substrates and is absolutely dependent on Ubc2/Rad6 orthologs [3].
  • Cyclization of polyubiquitin by the E2-25K ubiquitin conjugating enzyme [4].
  • We show that rabbit reticulocyte lysate harbors enzymatic components that catalyze the assembly of unanchored Lys-29-linked polyubiquitin chains [5].

Biological context of UBC

  • In this report, we present the results of analyzing the nucleotide sequence of polyubiquitin genes of mammals, available in the DDBJ/EMBL/GenBank nucleotide sequence databases, in which we found that there are two groups of polyubiquitin genes in an orthologous relationship [6].
  • Although the predicted amino acid sequence of the ubiquitin from E. histolytica differs significantly (in 7-9 amino acid residues) from that of yeast and animals, expression of the coding sequence of EhUBI1 suppressed the heat-sensitive phenotype of a polyubiquitin gene-deficient yeast mutant [7].

Associations of UBC with chemical compounds

  • E2/E3-mediated assembly of lysine 29-linked polyubiquitin chains [5].
  • Fluorescent labeling of the six cysteines in the ovomucoid moiety (OM) with Lucifer Yellow iodoacetamide yields UbOM(LY); the ubiquitin in the fusion protein can be extended by the addition of a K48-linked polyubiquitin chain to form Ub(n)OM(LY) [8].

Enzymatic interactions of UBC

  • The mammalian ubiquitin conjugating enzyme known as E2-25K catalyzes the synthesis of polyubiquitin chains linked exclusively through K48-G76 isopeptide bonds [9].

Analytical, diagnostic and therapeutic context of UBC

  • One of these, EhUBI1, was cloned and sequenced and found to correspond to a monoubiquitin gene; as shown by a polymerase chain reaction, E. histolytica lacked polyubiquitin genes altogether [7].


  1. Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Hofmann, R.M., Pickart, C.M. Cell (1999) [Pubmed]
  2. Identification of four genes in endothelial cells whose expression is affected by tumor cells and host immune status--a study in ex vivo-isolated endothelial cells. Liliensiek, B., Rocha, M., Umansky, V., Benner, A., Lin, J., Ziegler, R., Nawroth, P.P., Schirrmacher, V. Blood (1998) [Pubmed]
  3. N-end rule specificity within the ubiquitin/proteasome pathway is not an affinity effect. Baboshina, O.V., Crinelli, R., Siepmann, T.J., Haas, A.L. J. Biol. Chem. (2001) [Pubmed]
  4. Cyclization of polyubiquitin by the E2-25K ubiquitin conjugating enzyme. Yao, T., Cohen, R.E. J. Biol. Chem. (2000) [Pubmed]
  5. E2/E3-mediated assembly of lysine 29-linked polyubiquitin chains. Mastrandrea, L.D., You, J., Niles, E.G., Pickart, C.M. J. Biol. Chem. (1999) [Pubmed]
  6. Interspecific comparison in the frequency of concerted evolution at the polyubiquitin gene locus. Nenoi, M., Ichimura, S., Mita, K. J. Mol. Evol. (2000) [Pubmed]
  7. Characterization of ubiquitin genes and -transcripts and demonstration of a ubiquitin-conjugating system in Entamoeba histolytica. Wöstmann, C., Liakopoulos, D., Ciechanover, A., Bakker-Grunwald, T. Mol. Biochem. Parasitol. (1996) [Pubmed]
  8. Ubiquitin-ovomucoid fusion proteins as model substrates for monitoring degradation and deubiquitination by proteasomes. Yao, T., Cohen, R.E. Meth. Enzymol. (2005) [Pubmed]
  9. Core domain mutation (S86Y) selectively inactivates polyubiquitin chain synthesis catalyzed by E2-25K. Mastrandrea, L.D., Kasperek, E.M., Niles, E.G., Pickart, C.M. Biochemistry (1998) [Pubmed]
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