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Gene Review

UBA52  -  ubiquitin A-52 residue ribosomal protein...

Bos taurus

Synonyms: RPS27A, UBB, UBC
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Disease relevance of MGC127041


Psychiatry related information on MGC127041


High impact information on MGC127041

  • Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue [8].
  • These findings support a model in which an Mms2p/Ubc13p complex assembles novel polyubiquitin chains for signaling in DNA repair, and they suggest that UEV proteins may act to increase diversity and selectivity in ubiquitin conjugation [8].
  • Ubiquitin tag for sperm mitochondria [9].
  • In eukaryotes, ubiquitin (Ub)-dependent proteolysis is essential for bulk protein turnover as well as diverse processes including cell-cycle control, differentiation, antigen presentation, and the stress response [10].
  • Molecular conservation of 74 amino acid sequence of ubiquitin between cattle and man [11].

Chemical compound and disease context of MGC127041


Biological context of MGC127041


Anatomical context of MGC127041


Associations of MGC127041 with chemical compounds

  • Nor have assembly mechanisms or definitive functions been assigned to polyubiquitin chains linked through several other lysine residues of ubiquitin [21].
  • We observed a replacement of serine (133) by phenylalanine in the second ubiquitin repeat [15].
  • Iodination of tyrosine 59 of ubiquitin selectively blocks ubiquitin's acceptor activity in diubiquitin synthesis catalyzed by E2(25K) [22].
  • The activity of the isopeptidase is inhibited by iodoacetamide and Ub aldehyde [18].
  • One of these chain members (Ubq2) was purified from wheat and was demonstrated via amino acid sequence analysis of tryptic fragments to consist of two ubiquitin monomers joined via a glycine 76-->lysine 48 linkage [23].

Physical interactions of MGC127041

  • Recent studies have shown that selection of proteins for degradation by the ubiquitin system occurs most probably by binding to specific sites of the ubiquitin-protein ligase, E3 [24].
  • We previously showed that the bovine papillomavirus E1 protein is unstable and becomes resistant to ubiquitin-mediated degradation when tightly bound to cyclin E-cyclin-dependent kinase 2 (Cdk2) before the start of DNA synthesis [25].

Enzymatic interactions of MGC127041


Regulatory relationships of MGC127041


Other interactions of MGC127041

  • This reaction can be reconstituted using the ubiquitin-conjugating enzyme (E2) known as UbcH5A, a 120-kDa protein(s) that behaves as a ubiquitin-protein ligase (E3), and ubiquitin-activating enzyme (E1) [21].
  • Experimental conditions were established under which a fusion protein containing an amino-terminal fragment of cyclin B is ligated to ubiquitin only in extracts from M-phase but not from interphase cells [33].
  • Components of a system that ligates cyclin to ubiquitin and their regulation by the protein kinase cdc2 [33].
  • For each of the subgenomes, a large internal deletion was found together with an inserted sequence encoding part of ribosomal protein S27a fused to an N-terminally truncated ubiquitin monomer [34].
  • Indeed, a novel enzyme, E3Histone, which could conjugate ubiquitin to histones H1, H2A, H2B, H3, and H4 in vitro, was found [16].

Analytical, diagnostic and therapeutic context of MGC127041

  • The properties of these enzymes suggest that there may be distinct pathways for ubiquitin-ubiquitin ligation versus substrate-ubiquitin ligation in vivo [21].
  • Tryptic digestion of maleated ubiquitin yielded four peptide fragments that were resolved by molecular sieve chromatography and coded in order of decreasing chain length (MT-1, MT-2, MT-3, and MT-4) [14].
  • Using the 20-kDa ubiquitin-conjugating enzyme TaUBC7 from wheat, we have generated free, glycine 76-->lysine 48-linked multiubiquitin chains (Ubqn), and have individually purified Ubqn species (n < or = 6) by anion-exchange, high pressure liquid chromatography [23].
  • Tryptic digestion of intact Ub2 gave products consistent with the existence of an isopeptide linkage between the COOH terminus of one Ub and Lys-48 of the other; this structure was confirmed by sequence analysis of the unique Ub2 tryptic fragment [35].
  • Immunofluorescence microscopy was used to study the intracellular localization of ubiquitin [36].


  1. Ribosomal S27a coding sequences upstream of ubiquitin coding sequences in the genome of a pestivirus. Becher, P., Orlich, M., Thiel, H.J. J. Virol. (1998) [Pubmed]
  2. Chronic metabolic acidosis increases mRNA levels for components of the ubiquitin-mediated proteolytic pathway in skeletal muscle of dairy cows. Mutsvangwa, T., Gilmore, J., Squires, J.E., Lindinger, M.I., McBride, B.W. J. Nutr. (2004) [Pubmed]
  3. The structure of ubiquitinated histone H2B. Thorne, A.W., Sautiere, P., Briand, G., Crane-Robinson, C. EMBO J. (1987) [Pubmed]
  4. Small ubiquitin-related modifier-1 modification mediates resolution of CREB-dependent responses to hypoxia. Comerford, K.M., Leonard, M.O., Karhausen, J., Carey, R., Colgan, S.P., Taylor, C.T. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  5. Extracellular ubiquitin inhibits the TNF-alpha response to endotoxin in peripheral blood mononuclear cells and regulates endotoxin hyporesponsiveness in critical illness. Majetschak, M., Krehmeier, U., Bardenheuer, M., Denz, C., Quintel, M., Voggenreiter, G., Obertacke, U. Blood (2003) [Pubmed]
  6. Increase in ubiquitin-protein conjugates concomitant with the increase in proteolysis in rat skeletal muscle during starvation and atrophy denervation. Wing, S.S., Haas, A.L., Goldberg, A.L. Biochem. J. (1995) [Pubmed]
  7. Ubiquitin: a multifunctional regulatory protein associated with the cytoskeleton. Fried, V.A., Smith, H.T. Prog. Clin. Biol. Res. (1989) [Pubmed]
  8. Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Hofmann, R.M., Pickart, C.M. Cell (1999) [Pubmed]
  9. Ubiquitin tag for sperm mitochondria. Sutovsky, P., Moreno, R.D., Ramalho-Santos, J., Dominko, T., Simerly, C., Schatten, G. Nature (1999) [Pubmed]
  10. Editing of ubiquitin conjugates by an isopeptidase in the 26S proteasome. Lam, Y.A., Xu, W., DeMartino, G.N., Cohen, R.E. Nature (1997) [Pubmed]
  11. Molecular conservation of 74 amino acid sequence of ubiquitin between cattle and man. Schlesinger, D.H., Goldstein, G. Nature (1975) [Pubmed]
  12. Folding and stability of a tryptophan-containing mutant of ubiquitin. Khorasanizadeh, S., Peters, I.D., Butt, T.R., Roder, H. Biochemistry (1993) [Pubmed]
  13. A high pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry ion source for thermal stabilization of labile biomolecules. O'Connor, P.B., Costello, C.E. Rapid Commun. Mass Spectrom. (2001) [Pubmed]
  14. The complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells. Schlesinger, D.H., Goldstein, G., Niall, H.D. Biochemistry (1975) [Pubmed]
  15. Characterization of a full-length cDNA encoding a bovine four tandem-repeat ubiquitin. Wempe, F., Scheit, K.H. Biochim. Biophys. Acta (1993) [Pubmed]
  16. Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones. Liu, Z., Oughtred, R., Wing, S.S. Mol. Cell. Biol. (2005) [Pubmed]
  17. The biosynthesis of ubiquitin by parathyroid gland. Hamilton, J.W., Rouse, J.B. Biochem. Biophys. Res. Commun. (1980) [Pubmed]
  18. A ubiquitin C-terminal isopeptidase that acts on polyubiquitin chains. Role in protein degradation. Hadari, T., Warms, J.V., Rose, I.A., Hershko, A. J. Biol. Chem. (1992) [Pubmed]
  19. Activity of ubiquitin-dependent pathway in response to oxidative stress. Ubiquitin-activating enzyme is transiently up-regulated. Shang, F., Gong, X., Taylor, A. J. Biol. Chem. (1997) [Pubmed]
  20. Ubiquitinylation and ubiquitin-dependent proteolysis in vertebrate photoreceptors (rod outer segments). Evidence for ubiquitinylation of Gt and rhodopsin. Obin, M.S., Jahngen-Hodge, J., Nowell, T., Taylor, A. J. Biol. Chem. (1996) [Pubmed]
  21. E2/E3-mediated assembly of lysine 29-linked polyubiquitin chains. Mastrandrea, L.D., You, J., Niles, E.G., Pickart, C.M. J. Biol. Chem. (1999) [Pubmed]
  22. Iodination of tyrosine 59 of ubiquitin selectively blocks ubiquitin's acceptor activity in diubiquitin synthesis catalyzed by E2(25K). Pickart, C.M., Haldeman, M.T., Kasperek, E.M., Chen, Z. J. Biol. Chem. (1992) [Pubmed]
  23. Multiubiquitin chains linked through lysine 48 are abundant in vivo and are competent intermediates in the ubiquitin proteolytic pathway. van Nocker, S., Vierstra, R.D. J. Biol. Chem. (1993) [Pubmed]
  24. Post-translational addition of an arginine moiety to acidic NH2 termini of proteins is required for their recognition by ubiquitin-protein ligase. Elias, S., Ciechanover, A. J. Biol. Chem. (1990) [Pubmed]
  25. Nucleocytoplasmic shuttling of bovine papillomavirus E1 helicase downregulates viral DNA replication in S phase. Hsu, C.Y., Mechali, F., Bonne-Andrea, C. J. Virol. (2007) [Pubmed]
  26. Ubiquitin carrier protein-catalyzed ubiquitin transfer to histones. Mechanism and specificity. Pickart, C.M., Vella, A.T. J. Biol. Chem. (1988) [Pubmed]
  27. Core domain mutation (S86Y) selectively inactivates polyubiquitin chain synthesis catalyzed by E2-25K. Mastrandrea, L.D., Kasperek, E.M., Niles, E.G., Pickart, C.M. Biochemistry (1998) [Pubmed]
  28. Recognition of modified forms of ribonuclease A by the ubiquitin system. Dunten, R.L., Cohen, R.E. J. Biol. Chem. (1989) [Pubmed]
  29. Multiple forms of ubiquitin-protein ligase. Binding of activated ubiquitin to protein substrates. Lee, P.L., Midelfort, C.F., Murakami, K., Hatcher, V.B. Biochemistry (1986) [Pubmed]
  30. Isolation and sequence of an interferon-tau-inducible, pregnancy- and bovine interferon-stimulated gene product 15 (ISG15)-specific, bovine ubiquitin-activating E1-like (UBE1L) enzyme. Rempel, L.A., Francis, B.R., Austin, K.J., Hansen, T.R. Biol. Reprod. (2005) [Pubmed]
  31. Transfer RNA is required for conjugation of ubiquitin to selective substrates of the ubiquitin- and ATP-dependent proteolytic system. Ferber, S., Ciechanover, A. J. Biol. Chem. (1986) [Pubmed]
  32. Ubiquitin is involved in the in vitro insertion of monoamine oxidase B into mitochondrial outer membranes. Zhaung, Z.P., McCauley, R. J. Biol. Chem. (1989) [Pubmed]
  33. Components of a system that ligates cyclin to ubiquitin and their regulation by the protein kinase cdc2. Hershko, A., Ganoth, D., Sudakin, V., Dahan, A., Cohen, L.H., Luca, F.C., Ruderman, J.V., Eytan, E. J. Biol. Chem. (1994) [Pubmed]
  34. RNA recombination between persisting pestivirus and a vaccine strain: generation of cytopathogenic virus and induction of lethal disease. Becher, P., Orlich, M., Thiel, H.J. J. Virol. (2001) [Pubmed]
  35. A 25-kilodalton ubiquitin carrier protein (E2) catalyzes multi-ubiquitin chain synthesis via lysine 48 of ubiquitin. Chen, Z., Pickart, C.M. J. Biol. Chem. (1990) [Pubmed]
  36. Ubiquitin is a component of the microtubule network. Murti, K.G., Smith, H.T., Fried, V.A. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
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